Product Description
“Methods in Yeast Genetics” is a course that has been offered annually at Cold Spring Harbor Laboratory for the last thirty years. This is an updated edition of the course manual, which provides a set of teaching experiments, along with protocols and recipes for the standard techniques and reagents used in the study of yeast biology. Since the last edition of the manual was published (2000), revolutionary advances in genomics and proteomics technologies have had a significant impact on the field. This updated edition reflects these advances, and also includes new techniques involving vital staining, visualization of Green Fluorescent Protein, new drug resistance markers, high-copy suppression, Tandem Affinity Protein tag protein purification, gene disruption by double-fusion polymerase chain reaction, and many other recent developments.
Book Description
The yeasts have been important experimental organisms for more than 50 years. This volume contains over 100 selected papers, in sections with introductions that describe the process of discovery and the context and significance of the research. The selections include early classics as well as recent advances in areas such as signal transduction, membrane trafficking, protein turnover, and genomics. This book is designed as a guide for a literature-based course.
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Yeast Physiology and Biotechnology
Graeme M. Walker
Manufacturer: Wiley
ProductGroup: Book
Binding: Paperback
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ASIN: 0471964468 |
Book Description
Yeasts are the world's premier industrial micro-organisms. In addition to their wide exploitation in the production of foods, beverages and pharmaceuticals, yeasts also play significant roles as model eukaryotic cells in furthering our knowledge in the biological and biomedical sciences. In order for modern biotechnology to fully exploit the activities of yeasts, it is essential to appreciate aspects of yeast cell physiology. In recent years, however, our knowledge of yeast physiological phenomena has lagged behind that of yeast genetics and molecular biology. Yeast Physiology and Biotechnology redresses the balance by linking key aspects of yeast physiology with yeast biotechnology. Individual chapters provide broad and timely coverage of yeast cytology, nutrition, growth and metabolism - important aspects of yeast cell physiology which are pertinent to the practical uses of yeasts in industry. The final chapter reviews traditional, modern and emerging biotechnologies in which roles of yeasts in the production of industrial commodities and their value in biomedical research are fully discussed. Relevant aspects of classical and modern yeast genetics and molecular biology are fully integrated into the appropriate chapters. This up-to-date and fully referenced book is aimed at advanced undergraduate and postgraduate bioscience students,but will also prove to be a valuable source of information for yeast researchers and technologists.
Customer Reviews:
low value.......2006-09-24
I found this introduction to yeast physiology and biotechnology to be well written and enjoyable to read. That being said, I gave it only 2 stars because the price is absolutely ridiculous. The coverage of most topics is rather superficial (not always a bad thing in an overview-type text, but inexcusable in light of the exorbitant cost of this boook), and the material is rather dated (this being a paperback edition from 1998). I'd definitely recommend that those interested in yeast research check out a copy from a library, or buy it if you can find a used copy for around 25% of the new price, but otherwise it is just not worth it.
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Yeasts in Food: Beneficial and Detrimental Aspects
T., Ed. Boekhout
Manufacturer: Chapman & Hall/CRC
ProductGroup: Book
Binding: Hardcover
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ASIN: 0849319269 |
Book Description
Maximising the benefits of yeast while minimising their detrimental effects requires a thorough understanding of their complex characteristics and how these can best be manipulated. This book describes the enormous range of yeasts together with methods for detection, identification, and analysis. It then discusses spoilage yeasts, methods of control, and stress responses to food preservation techniques. Against this background, the book looks at the role of yeasts in particular types of food. Each chapter describes the diversity of yeasts associated with each type of food, their beneficial and detrimental effects on food quality, methods of analysis and quality control.
Book Description
Guide to Yeast Genetics and Molecular Biology presents, for the first time, a comprehensive compilation of the protocols and procedures that have made Saccharomyces cerevisiae
such a facile system for all researchers in molecular and cell biology. Whether you are an established yeast biologist or a newcomer to the field, this volume contains all the up-to-date methods you will need to study "Your Favorite Gene" in yeast.
* Basic Methods in Yeast Genetics
* Physical and genetic mapping
* Making and recovering mutants
* Cloning and Recombinant DNA Methods
* High-efficiency transformation
* Preparation of yeast artificial chromosome vectors
* Basic Methods of Cell Biology
* Immunomicroscopy
* Protein targeting assays
* Biochemistry of Gene Expression
* Vectors for regulated expression
* Isolation of labeled and unlabeled DNA, RNA, and protein
Customer Reviews:
A good book for those who work with budding yeast.......2000-10-25
This is a thorough book on yeast genetics (mainly budding yeast). It gives a vast background on most basic techniques. This includes the basic methods used in the field with some background material. Additional topics are covered (e.g. Ð cloning and recombinant DNA, mutants production, gene expression, as well as topics in cell biology and transport). Those who look for protocols for fission yeast, this is not the book.
A good book for those who work with budding yeast.......2000-10-03
This is a thorough book on yeast genetics (mainly budding yeast). It gives a vast background on most basic techniques. This includes the basic methods used in the field with some background material. Additional topics are covered (e.g. Ð cloning and recombinant DNA, mutants production, gene expression, as well as topics in cell biology and transport). Those who look for protocols for fission yeast, this is not the book.
Book Description
From a to á is a short supplemental textbook that uses control of yeast mating type as a model for many aspects of cell determination in general. Topics covered include gene silencing; genetic recombination; differentiation; combinatorial gene regulation; mRNA transport to establish asymmetric cell division; signal transduction; evolution of genetic networks; and various aspects of cell biology, including action of cytoskeleton and bud site selection. The book includes a foreword by Mark Ptashne, author of A Genetic Switch.
Customer Reviews:
Starting from the Basics.......2007-03-11
Complex organisms such as humans are made up of many different cell types. Understanding how these cell types differ and how they arise from undifferentiated embryonic stem cells is a major challenge for modern biologists. But even the single-celled baker's yeast can exist as any one of three distinct cell types, and our understanding of these different forms illuminates the key principles that underlie the programming and properties of cell types in higher organisms.
This book is about this yeast and presents these principles in a single, concise narrative. Topics covered include combinatorial gene regulation, gene silencing, DNA rearrangements, evolution of genetic networks, mRNA localization is asymmetric cell division, protein secretion mechanisms, and the role of the cytoskeleton in cell polarization.
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Growth, Differentiation and Sexuality (The Mycota) (MYCOTA, THE)
U., Ed. Kues
Manufacturer: Springer
ProductGroup: Book
Binding: Hardcover
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ASIN: 3540281347 |
Product Description
Since publication of the first edition of Volume I in 1994, the field of fungal biology has developed tremendously, mainly through the advancement of various molecular techniques and international fungal genome projects. To accommodate these developments, the second edition has been completely updated. Six chapters have been revised by former authors, others by newly recruited experts, and also novel subjects, emerged in more recent years, have been added to the book. Leading scientists in the field have compiled comprehensive overviews as well as latest results obtained from cytological, genetic and molecular studies. Topics include: cellular and colony growth of fungi, cellular fusion and incompatibility, senescence and programmed cell death, environmental and physiological signalling in differentiation processes, asexual and sexual reproduction, mitosis and meiosis of various types of fungi. Both parallels and differences become visible between individual fungi as well as between fungal classes.
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Yeast Stress Responses (Topics in Current Genetics)
S. Ed. Hohmann
Manufacturer: Springer-Verlag
ProductGroup: Book
Binding: Hardcover
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ASIN: 3540439269 |
Book Description
Every cell has developed mechanisms to respond to changes in its environment and to adapt its growth and metabolism to unfavorable conditions. The unicellular eukaryote yeast has long proven as a particularly useful model system for the analysis of cellular stress responses, and the completion of the yeast genome sequence has only added to its power
This volume comprehensively reviews both the basic features of the yeast genral stress response and the specific adapations to different stress types (nutrient depletion, osmotic and heat shock as well as salt and oxidative stress). It includes the latest findings in the field and discusses the implications for the analysis of stress response mechanisms in higher eukaryotes as well.
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Molecular Biology of Schizosaccharomyces Pombe: Genetics, Genomics and Beyond
Richard Ed. Egel
Manufacturer: Springer-Verlag
ProductGroup: Book
Binding: Hardcover
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ASIN: 3540006931
Release Date: 2007-05-25 |
Product Description
The fission yeast Schizosaccharomyces pombe is the favoured tool of many productive research groups throughout the world, serving as a useful model for fundamental principles and mechanisms, such as genome organization, differential gene regulation, cell-cycle control, signal transduction, or cellular morphogenesis. This book collates the current state of knowledge derived from molecular studies in this simple eukaryotic microorganism. The entire sequence of its genome has been completed, emphasizing the comparative value and model status of this yeast. The individual chapters, highlighting up-to-date views on prominent aspects of molecular organization, were written by active research scientists, presenting the results of their investigations to other workers in neighbouring fields. This book intends to serve the fission yeast community as a handy source of reference for years to come. It will also be of particular value to the ever-increasing number of researchers starting to look into fission yeast affairs for comparative reasons from other platforms of molecular genetics and cell biology.
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Yeast Protocols (Methods in Molecular Biology) (Methods in Molecular Biology (Cloth))
Wei, PhD Xiao
Manufacturer: Humana Press
ProductGroup: Book
Binding: Hardcover
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Methods in Yeast Genetics: A Cold Spring Harbor Laboratory Course Manual, 2005 Edition (Cold Spring)
ASIN: 1588294374 |
Book Description
In this second edition of a widely used classic laboratory manual, leading experts utilize the tremendous progress and technological advances that have occurred to create a completely new collection of not only the major basic techniques, but also advanced protocols for yeast research and for using yeast as a host to study genes from other organisms. The authors provide detailed methods for the isolation of subcellular components-including organelles and macromolecules, for the basic cellular and molecular analysis specific for yeast cells, and for the creation of conditional mutant phenotypes that lend themselves to powerful genome manipulation. Additional protocols offer advanced approaches to study genetic interactions, DNA and chromatin metabolism, gene expression, as well as the foreign genes and gene products in yeast cells.
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