Gene Knockout Protocols (Methods in Molecular Biology (Cloth))

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Highly skilled investigators with extensive experience in gene targeting and mouse genetics describe their best techniques for the design of targeting constructs and for the analysis of the mouse phenotype. These include embryo transplantation, in vitro embryonic stem cell differentiation, creation of aggregation chimeras, mouse pathology, embryo cryopreservation, and transplantation. State-of-the-art and highly practical, Gene Knockout Protocols not only constitutes an invaluable source of readily reproducible techniques for those just entering the field of gene targeting, but also a key reference for all genetic researchers today.

Polymer Therapeutics I: Polymers as Drugs, Conjuates and Gene delivery Systems (Advances in Polymer Science)

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Polymer Therapeutics I: Polymers as Drugs, Conjuates and Gene delivery Systems (Advances in Polymer Science)
Satchi-Fainaro R.
Manufacturer: Springer
ProductGroup: Book
Binding: Hardcover

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ASIN: 3540292101

Cytokine Gene Polymorphisms in Multifactorial Conditions

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Cytokine Gene Polymorphisms in Multifactorial Conditions
Koen Vandenbroeck
Manufacturer: CRC Press
ProductGroup: Book
Binding: Hardcover

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ASIN: 0849336198

Book Description

The literature on cytokine genetics is vast, so vast that it is now practically beyond the time or logistical constraints of most scientists to successfully keep pace with it. A compilation of the latest research, Cytokine Gene Polymorphisms in Multifactorial Conditions brings together, reviews, and structures up-to-date information on polymorphisms in cytokine genes. It discusses haplotype structures and linkage disequilibrium patterns in cytokine gene loci; functional biological effects of polymorphisms; and genetic associations with disease. The book documents polymorphisms in the most important cytokine genes, or gene clusters, and their biological and genetic effects in a multitude of distinct multifactorial conditions. Unique to this book are the "disease-centered" chapters examining the role of cytokine gene polymorphisms in a multitude of multifactorial conditions. The conditions include autoimmune or chronic inflammatory diseases, cardiovascular disease, infectious diseases, and longevity. "This section is a real tour de force" (Grant Gallagher and Michael F. Seldin, March 2006). Broadening the understanding of the effect of genetic variations on human immune responses, the organization, scope, and content of this book make it a valuable and easily accessible resource. The book integrates genetic, immunological, and clinical information and will serve as a reference for novice and expert geneticists, immunologists, cell biologists and clinicians. It is a must for everyone involved in, or planning, cytokine genetics or immunogenetics studies.

Laboratory Protocols For Conditional Gene Targeting

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Laboratory Protocols For Conditional Gene Targeting
Raul M. Torres , and Ralf Kuhn
Manufacturer: Oxford University Press
ProductGroup: Book
Binding: Hardcover

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Manipulating the Mouse Embryo: A Laboratory Manual

ASIN: 019963677X

Book Description

Conditional gene targeting is an emerging technique for introducing mutations into the genes of laboratory mice. This collection of protocols comes from one of the world's premier laboratories for gene targeting The Institute of Genetics in Cologne and is intended for newcomers to the field as well as more experienced researchers. Topics covered include: the Cre/loxP recombination system and gene targeting in ES cells; gene targeting strategies; Cre/loxP recombination system; Gene targeting vectors consideration and use of loxP; nonselectable modifications and removal of selection marker genes; large deletions; gene replacement; chromosomal translocations; targeted integrations; conditional gene modification; LoxP-containing transgenes; conditional gene targeting; working with ES cells; ES cell transfection overview; embryonic feeder cells; ES cells handling and use; ES cell culture and transfection; ES clone picking; identification of homologous recombinants; freezing ES clones; thawing ES clones; genomic DNA preparation; cre-mediated neomycin deletion in ES cells; generation of mice preparation; mouse breeding for ES cell injection or aggregation; injection needles and holders; blastocyst preparation/injection; embryo aggregation; blastocyst transfer;

Understanding Gene Therapy (MEDICAL PERSPECTIVES SERIES)

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The vital facts about the dangers of gene therapy.
Fantastic book on understanding gene therapy

Understanding Gene Therapy (MEDICAL PERSPECTIVES SERIES)
N., Ed. Lemoine
Manufacturer: Springer
ProductGroup: Book
Binding: Paperback

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ASIN: 0387915125

Book Description

What is gene therapy? How are genes delivered? Which diseases can be treated? What is the status of current clinical trials? What are the ethical issues? What are the future prospects? Understanding Gene Therapy answers all these questions and more, providing an ideal introduction to this complex and rapidly developing field. Written and edited by leading experts, the book provides a highly readable and comprehensive overview of the subject and is clearly and simply illustrated in two colors throughout.

Understanding Gene Therapy is a suitable introductory text for medical students and clinicians, and essential reading for genetics students, and researchers.

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The vital facts about the dangers of gene therapy........2006-09-02

In Nicholas Lemoine's book: Understanding Gene Therapy, he poses a simple idea but one with enormous potential. If a gene is defective in the human body, just replace it with one that works properly. Gene therapy would mean that genetic disorders would become a thing of the past. Cancer would be cured, as would cystic fibrosis and hundreds of other genetic illnesses. Although the concept of gene therapy is simple, the practice of administering the treatment is much more difficult. In order to replace defective genes, doctors must get working ones into the body and to the place where they are needed. Scientists had an ingenious solution to the problem. Hijack one of our most deadly enemies--the virus. A virus infects a human by inserting its harmful genes directly inside our cells. Normally this causes damage to the cell making us sick, but scientists were convinced they could tame this natural ability. Replace the harmful genes with good ones, and the virus would be the perfect delivery vehicle. It was initially believed that a retrovirus would be the best way of getting modified genes into the body. Most viruses and cellular organisms store their genetic material as DNA. Retroviruses use RNA instead. Retroviruses combine their genetic material with that of the host permanently, hence they can offer a permanent cure.

Unfortunately trials using retroviruses had very little success treating any illness other than one particular type of blood disorder, severe combined immunodeficiency. Scientists realized that if they wanted to treat organs in the body like the heart, liver or lungs, they would have to find an alternative way of delivering their treatment--retroviruses simply couldn't get in.

The answer would come in 1992. Using the most common virus around: the adenovirus, cause of the common cold, seemed like a good choice because it is able to affect almost every cell in the human body. But Scientists needed a disease to try the adenovirus vector on, and set their sights on OTC deficiency. So in 1998 the "subject" was approached to participate in a trial. He knew that taking part was not going to cure his OTC but he was keen to help because he knew that if successful he would have played a part in curing thousands of diseases. Later that year he arrived at the University of Pennsylvania to begin the trial. One day later a scientist injected him with the tame viral particles. He was given the biggest adenovirus dose of any of the trial participants. The doctors told him to expect a small reaction to the virus. Two days later, when a nurse checked on him, she found that he was slightly confused and jaundiced. Although they doubted this was serious, the team wanted to be sure. The adenovirus was supposed to be harmless but his body was behaving like it was under attack. By the next day he was in a coma. Over the next two days his condition deteriorated until on day five, the team of doctors delivered the devastating news to his parents. He had no brain activity, and his internal organs were shutting down. The doctors suggested that he be removed from life support. He died a day later. What chance of success is there now for gene therapy and at what risk? For many people the dream of gene therapy is dead. It is not the cure all that was dreamed of years ago, but instead it is a highly selective treatment for a very small number of diseases. Gene therapy may still become an effective treatment for thousands of people but this is no miracle cure.

Fantastic book on understanding gene therapy.......2001-01-03

This was a great book, and gave the truth about the pros and cons of gene therapy and the progress that has been achieved. This book is appropriate for undergrads with a strong science background, medical students, science graduate students, and physicians and researchers and would be very important reading on this quickly emerging field. Included are some nice diagrams and some up-to-date key references in the field that will encompass the future of medicine.

Gene Targeting and Embryonic Stem Cells (Advanced Methods (BIOS))

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Gene Targeting and Embryonic Stem Cells (Advanced Methods (BIOS))
J. Ed. McWhir
Manufacturer: BIOS Scientific Publishers
ProductGroup: Book
Binding: Paperback

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Gene Targeting: A Practical Approach

ASIN: 1859963609

Book Description

The Advanced Methods series is intended for advanced undergraduates, graduate students and established research scientists. Titles in the series are designed to cover current important areas of research in life sciences, and include both theoretical background and detailed protocols. The aim is to give researchers sufficient theory, supported by references, to take the given protocols and adapt them to their particular experimental systems.

The recent isolation of human embryonic stem cells and the potential of these cells for therapeutic applications has generated an entirely new methodology. Similarly, gene targeting methodology has recently been extended to nuclear donor cells in ungulate species. This volume will be invaluable for both new and established researchers in the field of human embryonic stem cells, and to biotech companies engaged in the production of transgenic proteins in livestock, xenotransplantation and the development of animal models.

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Nonviral Vectors for Gene Therapy

Manufacturer: Academic Press
ProductGroup: Book
Binding: Hardcover

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ASIN: 0123584655

Book Description

Gene transfer within humans has been an obstacle until about 10 years ago. At that time, it was found that viral vectors were effective carriers of "healthy genes" into patients' cells. The problem, however, was that viral vectors proved unnecessarily harmful to humans: subjects experienced inflamatory activity and negative immunological responses to the genes. Viral vectors were also unable to meet the needs of the pharmaceutical community: they were not reproducible in large-scale proportions in cost-effective ways.
Thus, research was undertaken to find a safer way to transfer genes to patients without jeopardizing the safety of the patient. And so non-viral vectors were discovered. This volume presents the various non-viral vectors currently under development. Although not methodologically oriented, it will provide the necessary details behind the development of the vectors. This information will prove useful to both researchers and clinicians.

Key Features
* Presents state-of-the art developments of nonviral vectors as tools for modern molecular medicine
* Covers all types of nonviral vectors, from molecular structure to therapeutic application
Provides a comprehensive review of synthetic vectors
* Includes contributions from major investigators and leading experts in the field

Gene Targeting: A Practical Approach (Practical Approach Series)

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Gene Targeting: A Practical Approach (Practical Approach Series)

Manufacturer: Oxford University Press, USA
ProductGroup: Book
Binding: Paperback

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ASIN: 0199634068

Book Description

The book begins by describing in detail the rationale behind designing gene targeting vectors, including both basic replacement vectors for creating null mutations and more sophisticated two-step approaches for creating subtle mutations. It describes the latest techniques for culturing and
manipulating ES cells and a number of approaches for identifying rare targeted cell clones. Techniques for culturing and assaying for mouse and human hematopoietic progenitors in vitro and in vivo are then covered, followed by the design of, and use of, retrovirus vectors for introducing and
expressing genes in bone marrow cells. Two approaches for making ES cell chimeras are described. One chapter covers the more standard approach of ES cell injection into blastocysts as well as morula injection. Another chapter describes techniques for making aggregation chimeras and in particular
a recent approach using tetraploid host embryos to produce completely ES cell-derived embryos and mice. The critical steps for obtaining germline chimeras are discussed. The book is concluded by a discussion of the many uses of enhancer, gene and promoter trap vectors, the advantages of each, and
describes in detail the techniques for producing and analyzing "trap" vector insertions in ES cells. The protocols are written with sufficient detail for a novice in the area to use them as a manual to learn the various techniques required for producing mutant mice, beginning with the embryonic
stem cells and ending up with chimeric mice for breeding.

Advanced Gene Delivery: From Concepts to Pharmaceutical Products (DRUG TARGETING AND DELIVERY)

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Advanced Gene Delivery: From Concepts to Pharmaceutical Products (DRUG TARGETING AND DELIVERY)
Alain, Ed. Rolland
Manufacturer: CRC
ProductGroup: Book
Binding: Hardcover

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ASIN: 9057024381

Book Description

A practical resource for everyone involved in the gene therapy field and in the design of effective gene delivery systems, this volume presents an overview and update of recent advances in the field of non-viral methods for the in vivo transfer of therapeutic genes to biological targets using conventional routes of administration. Methods to control the spatial and temporal modulation of gene function in vivo as well as the level, duration, specificity, and fidelity of gene expression are described. The rational design and the applications of a variety of non-viral gene delivery systems, such as cationic lipid-, polymer-, and (poly) peptide-based systems, are exemplified for the control of location of therapeutic genes administered by various routes. Current and potential clinical applications of gene-based medicines are presented for the prevention, correction or modulation of diseases. Examples of current applications of plasmid-based systems for genetic vaccination, treatment of genetic disorders such as cystic fibrosis, and treatment of acquired diseases such as cancer are also provided.

Cytokine Knockouts (CONTEMPORARY IMMUNOLOGY)

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Cytokine Knockouts (CONTEMPORARY IMMUNOLOGY)
Giamila, Ed. Fantuzzi
Manufacturer: Humana Press
ProductGroup: Book
Binding: Hardcover

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ASIN: 1588291944

Book Description

Carrying on the high standards of the much praised first edition (Durum and Muegge, Cytokine Knockouts, 1998), Giamila Fantuzzi and a panel of experts have generated completely new chapters to reflect the use of many novel mouse strains and the hundreds of recent studies on cytokine physiology. Comprehensive reviews of the numerous often-surprising results obtained using cytokine knockout mice are provided, along with much important information about cytokine biology and physiology. For those not familiar with cytokine research, the authors present a critical discussion of the advantages and disadvantages of using cytokine knockout mice in various fields of research.

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