Protocols for Gene Analysis (Methods in Molecular Biology (Cloth))

Book Description

This volume provides a set of protocols for the analysis of cloned genes. A set of techniques is described for the basic manipulation and mutagenesis of the cloned sequences. The rest of the volume is organized into a collection of techniques appropriate to the analytical route to be investigated; these include genome structure, sequence variation, gene expression, and protein function. Finally, a number of methods are described to take the step from the first gene to those with which it interacts.

2-D Proteome Analysis Protocols (Methods in Molecular Biology (Cloth))

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2-D Proteome Analysis Protocols (Methods in Molecular Biology (Cloth))
Andrew, J. Link
Manufacturer: Humana Press
ProductGroup: Book
Binding: Hardcover

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ASIN: 0896035247

Book Description

A step-by-step tour through the complete process of doing proteomics. With easy-to-follow instructions, complete with many helpful hints and explanations, leading investigators and pioneers in the field show how to make protein extracts, reproducibly run them on 2-D gels, detect them, analyze the data, and precisely identify each protein. The book covers the latest methods of using carrier ampholytes in the 1st dimension, casting and running immobilized pH gradient 2-D gels, MALDI-TOF-based peptide mapping, automated tandem mass spectrometry, and nanoelectrospray ionization technology. For the 2nd dimension, there are methods for running flatbed or vertical gels and for protein detection using autoradiography, and Coomassie, silver, and reversible metal-chelate stains. 2-D Proteome Analysis Protocols is the most complete guide for using proteomics to answer biological questions.

Adrenergic Receptor Protocols (Methods in Molecular Biology (Cloth))

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Adrenergic Receptor Protocols (Methods in Molecular Biology (Cloth))
Curtis A., Ed. Machida
Manufacturer: Humana Press
ProductGroup: Book
Binding: Hardcover

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ASIN: 0896036022

Book Description

Curtis Machida and a panel of expert investigators present a comprehensive collection of modern molecular methods for analyzing adrenergic receptors and corresponding second messenger systems. These proven and readily reproducible techniques utilize genetic, RNA, protein expression, transactivator, and second messenger methodologies, as well as immunocytochemical, electrophysiological, transgenic, and in situ hybridization approaches. Each of the experts writing here details the use of their chosen method in examining the adrenergic receptor system, using aspects of the genetic flow of information as a guide: DNA ' RNA ' transactivator ' protein expression ' second messenger analyses ' cellular analyses ' transgenic whole animal approaches. Comprehensive and rich in practical detail, Adrenergic Receptor Protocols provides the first collection of readily reproducible methods for the study of these important regulators of CNS-mediated behavior and neural functions.

Methods in Enzymology Vol 410: DNA Microarrays, Part A: Array Platforms and Wet-Bench Protocols (Methods in Enzymology)

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Methods in Enzymology Vol 410: DNA Microarrays, Part A: Array Platforms and Wet-Bench Protocols (Methods in Enzymology)
Alan R., Ed. Kimmel
Manufacturer: Academic Press
ProductGroup: Book
Binding: Hardcover

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Methods in Enzymology, Vol 411: DNA Microarrays, Part B: Databases and Statstics (Methods in Enzymology)

ASIN: 0121828158

Book Description

Modern DNA microarray technologies have evolved over the past 25 years to the point where it is now possible to take many million measurements from a single experiment. These two volumes, Parts A & B in the Methods in Enzymology series provide methods that will shepard any molecular biologist through the process of planning, performing, and publishing microarray results.

Part A starts with an overview of a number of microarray platforms, both commercial and academically produced and includes wet bench protocols for performing traditional expression analysis and derivative techniques such as detection of transcription factor occupancy and chromatin status. Wet-bench protocols and troubleshooting techniques continue into Part B. These techniques are well rooted in traditional molecular biology and while they require traditional care, a researcher that can reproducibly generate beautiful Northern or Southern blots should have no difficulty generating beautiful array hybridizations.

Data management is a more recent problem for most biologists. The bulk of Part B provides a range of techniques for data handling. This includes critical issues, from normalization within and between arrays, to uploading your results to the public repositories for array data, and how to integrate data from multiple sources. There are chapters in Part B for both the debutant and the expert bioinformatician.

· Provides an overview of platforms
· Includes experimental design and wet bench protocols
· Presents statistical and data analysis methods, array databases, data visualization and meta analysis

Reviews and Protocols in DT40 Research (Subcellular Biochemistry)

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Reviews and Protocols in DT40 Research (Subcellular Biochemistry)
Jean-Marie, Ed. Buerstedde
Manufacturer: Springer
ProductGroup: Book
Binding: Hardcover

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Book Description

Research is fast paced and advances in RNA interference have recently opened up new opportunities for genetic experiments in human cell lines. However, the possibility to easily modify the genome still remains a powerful tool to investigate the function of coding and regulatory sequences in the vertebrate genome. DT40 has never been a quick and easy road to fame. It would be unfair to blame this on DT40 as it has proven to be a reliable and robust companion with fast doubling time, easy clonability and a relatively stable karyotype. If this model system is going to flourish over the next 15 years, it will be thanks to ingenious and original researchers. They may feel as if they work outside the mainstream, but they can take heart by the fact that only the clever exploitation of diversity and conservation makes biological research both elegant and rewarding. It is with this in mind that the DT40 handbook has been conceived and written. This book provides an up to date overview of the different facets of research, and also intends to help newcomers get started and avoid looming pitfalls. The collection of protocols which have been kindly provided by a number of laboratories will be particularly useful in this regard.

Serial Analysis of Gene Expression (SAGE): Ditigal Gene Expression Profiling (Methods in Molecular Biology (Cloth))

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Serial Analysis of Gene Expression (SAGE): Ditigal Gene Expression Profiling (Methods in Molecular Biology (Cloth))
Kare Lahmann Nielsen
Manufacturer: Humana Press
ProductGroup: Book
Binding: Hardcover

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Book Description

Serial Analysis of Gene Expression: Methods and Protocols facilitates the introduction of SAGE into the laboratory, and provides a framework for interpreting and comparing data derived from SAGE experiments. Of the several methods of genetic profiling available, only SAGE measures the expression of both known and unknown genes. SAGE studies encompass 50,000 tags and can provide detailed knowledge of the 2000 most highly expressed genes in the tissue sample. The SAGE protocols presented are detailed, fully annotated, and tested, and are all written by experienced SAGE researchers from around the world. Part 1 is dedicated to experimental procedures of SAGE and related methods including aRNA LongSAGE, SuperSAGE, DeepSAGE, and GMAT. Part 2 provides methods for extraction and filtration of tags, analysis of ditag populations, and completing statistically correct comparisons of gene expression profiles.

Book Description

The Greatest Fly Fishing Around the World is an anthology covering the sport of fishing from all parts of the planet. With over 300 stunning, full-color photographs, readers are taken along on thrilling trips fishing for trout, salmon, tarpon, bonefish, and permit. Includes inspirational fishing tales from some of the world’s greatest fishermen, including Clive Gammon, John Gierach, Brian Clarke, Ernest Schwiebert, Leonard M. Wright Jr., Tom McGuane, and many more.

The Greatest Fly Fishing Around the World (Greatest)

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A must for all traveling fly fishers
The Greatest fly Fishing Around Rhe World
A Beauty!!
Two books in one

The Greatest Fly Fishing Around the World (Greatest)
R. Valentine Atkinson
Manufacturer: The Lyons Press
ProductGroup: Book
Binding: Hardcover

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ASIN: 1592280862

Book Description

The Greatest Fly Fishing Around the World sweeps readers along on a worldwide journey to experience the beauty and thrill of fishing for trout, salmon, tarpon, bonefish, and permit. With more than 300 stunning color photographs from the highly individualized lens of R. Valentine Atkinson, and twenty-three stories from contemporary and classic fly-fishing writers, it captures all the irresistible visual and literary facets of the sport that continues to inspire millions of enthusiasts all over the world. The fly-fishing destinations were chosen for the interest and variety of their fishing, and for the impact and beauty of their landscapes--from quietly gurgling streams and secretive river pools to the turquoise seas off the Florida Keys. The collection includes stories from:

Clive Gammon o John Gierach o Brian Clarke o Ernest Schwiebert o Leonard M. Wright, Jr. o Tom McGuane o Roderick Haig-Brown o Peter Kaminsky o J. W.Hills o Neil Patterson o Russell Chatham o Art Lee o Nick Lyons o David Street o Zane Grey o Verlyn Klinkenborg o David Profumo

Through their pens, and through Val Atkinson's lens, we travel to the vast wilderness of Alaska, the tweedy moors of Scotland, the languid heat of the Caribbean, the chalk streams of southern England, the rocky grandeur of South Island in New Zealand, and many others. A celebration of the ancient art, as well as an encapsulation of every fly-fisherman's dreams, this is a unique and essential addition to the library of every fly-fishing enthusiast.

Customer Reviews:

A must for all traveling fly fishers.......2005-10-14

The photographs are beautiful and the text just enough without being too much. Good descriptions of the places around the world that are covered and an excellent fact page at the end of each section with information specific to fishing in that area on the fish, rods, flies, weather, accommodations, best fishing seasons, etc. A great book.

The Greatest fly Fishing Around Rhe World.......2005-10-03

The prefect gift for a fly fisherman/woman.

A Beauty!!.......2005-08-29

Here's a wonderful coffeetable style book that combines great photography with information. Even your nonflyfishing friends will love thumbing through the book. If you love flyfishing then you need this book

Two books in one.......2003-11-27

Beatiful book - beatiful print - beatiful pictures - the best.
Be aware though - the content is "Distant Waters" and "Trout and Salmon" put together in one book...

Book Description

This powerful book builds on the success of it predecessor-Writing the Breakout Novel-and takes readers to the next level: improving their work with practical, hands-on lessons and exercises.

With patented techniques from his popular writing workshops, Donald Maass shows fiction writers exactly how to make their work stand out from the crowd. Readers will learn how to strengthen any novel-in-progress, with sections on: - Building Plot Layers - Creating Inner Conflict - Strengthening Point of View and Voice - Discovering Larger Than Life Character Qualities - Developing the Psychology of Place

Every workbook exercise is followed by examples from real-life breakout novels. This is one of the most accessible novel-writing guides available today.

Customer Reviews:

I use this more than the actual book.......2007-10-08

I have both workbook and book. I use this when I'm plotting or replotting a book. I find the exercises make me think hard about my stories and bring out my creativity. I'd definitely recommend it.

Helpful!.......2007-08-24

I finally bought this book after rechecking it from the library several times. I love the challanges presented and the advice given. This book has helped me dig deeper and develop my own novel.

Not the same as the Breakout book.......2007-03-14

Since he published Writing the Breakout Novel in 2001, Maass has taught a seminar of the same name at many writers conferences, and naturally learned from these experiences. The original book is here expanded by nearly three dozen exercises, which seem quite sensible, not hokey as are some by other teachers I've read.

I own the Breakout book and just now am comparing the workbook that I checked out of the local library. They're not the same, not by a long shot, despite what one reviewer here says. Though many sections have identical headers (such as Inner Conflict), they are completely rewritten, using different examples. The original book is 259 pages of 6x9, the workbook is 230 pp. of 8-1/2x11 format, thus it is by no means a condensation as TheCafeWriter asserts, and the original book is not necessarily more "in-depth." Some sections are, some aren't. The structure is substantially rearranged. Yet the concepts and the really fundamental points -- keep your story charged with tension, and so on -- do remain identical. These are essentially two complementary treatments of the same material by the same author

Maass asks his seminar participants to bring their in-process novels to perform exercises on, so the workbook is particularly useful if you are well into writing a novel already. With the discipline of the exercises, Maass teaches you to be your own draft doctor.

For me, there appear to be easily enough new perspectives and ideas here to warrant buying the workbook even though I have read the original and have it on my bookshelf.

Good Hands On Exercises.......2006-08-14

This book is most beneficial to writers who have already completed the first drafts of their manuscripts. The exercises made me review some of my characters' attributes and turn offs as well as making sure my secondary plots tied into my main plot. Mr. Maass' suggestions caused me to re-think my villians a little bit and show a side to them that could be somewhat empathetic. Also, adding tension on every page for me was tough, but I found creative ways to do it. I do believe my completed novel is better now after making suggested changes. I stuck to my own gut on some of the things he suggested I change, but overall I did use a lot of his advice. This is a good purchase but you must do the written exercises not just skim over them.

It's good, but..........2006-04-01

Maass has created a good workbook with some nifty ways to come up with information. He's also included some material that's new, especially in terms of how to put together plots and subplots, and he expands the theme section from Writing the Breakout Novel.

Two problems though: first, except for a few places, the workbook really retreads the original book, just barely expanding on the original. I was hoping for more nuggets of information.

The second problem is that it is a workbook. I hate workbooks, mostly because my brain simply doesn't do those kinds of questions. I work from story down, not parts of story up. The workbook gives the impression to writers that answering the questions will magically make the story fall into place, and I instinctively reject that attitude. No, Maass isn't deliberately trying to tell writers the process, but the impression is there.

I would have rather he put together a follow-up book and then listed these questions at the end of the chapters instead of formatting the book with lines to be filled in. I think I would have been more receptive to the content. As it was, I read hastily through, feeling nauseous that I wasn't doing my homework--a kid's attitude from far too many workbooks in grade school.

Basically, good but not great information, and formatting that puts me off means that I have a hard time recommending this book. But if you're a workbook type, go for it.

Book Description

This book will help you take a badly wounded patient to the operating room, organize yourself and your team, do battle with some vicious injuries, and come out with the best possible result. It is a practical guide to operative trauma surgery for residents and registrars, for general surgeons with an interest in trauma, and for isolated surgeons operating on wounded patients in military, rural, or humanitarian settings. A surgical atlas may show you what to do with your hands - but not how to think, plan, and improvise. Here you will find practical advice on how to use your head as well as your hands when operating on a massively bleeding trauma patient.

Customer Reviews:

Superb "how to act in emergencies" book.......2007-04-10

This book, subtitled "The art & craft of trauma surgery" is a gem. Worth every penny (or whatever coins you spend). A very practical book on how to handle major trauma of all organ systems, which first directs your thoughts and then your hands.

Vintage Taub.......2006-08-20

I thoroughly enjoyed this book not for the "how to do it"aspects of the book but for the "why to do it this way at this point in time" aspect. As the authors point out, much of trauma surgery is time critical, and options are often limited. Surgical decision making at this juncture is the factor that often leads to success or failure - Hirshberg and Mattox succinctly list options and the reasons for following one line of attack instead of another given different circumstances. The book is almost flippant in its language on many occasions, and for those of us who have done time at Ben Taub know that this is not in character for either author, for whom the art and craft of surgery is deadly serious. The apparent flippant tone introduced into the book allows the authors to provide emphasis in a way that a chart or graph wouldn't - fluently and with humour. The book is presented in the way that a mentor may review a case in the surgeons lounge - simply, clearly, and with relevance. This is an extremely useful book for those involved with trauma surgery, and I hope to see new editions of this book at regular intervals.

Uma boa ideia.......2005-09-23

Um bom trabalho, livro de fácil entendimento e leitura. Lamento apenas a falta de detalhamento em vias aéreas.

top knife.......2005-08-27

overall a good book, with many practical tips. illustations are also good. i found the colloquial style irritating. ie. "The distal pancreas can be easily resected, while the pancreatic head requires a very big whack." These sort of practical manuals are valuable to practicing surgeons who know how to operate but can improve outcomes with the thinking portion of the operation, the art and craft of surgery.

Top Knife is a Top Book.......2005-08-16

Truly outstanding book for anyone providing surgical care to trauma patients. We've decided to purchase a copy for each of our senior residents so they will definitely read it. Easy to read. Practical knowledge and techniques, evidence based where possible, experienced base always. It reinforced much I've learned in 25 years of trying to teach trauma surgery and taught me new tricks and concepts.

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