Manipulating the Mouse Embryo: A Laboratory Manual
Average customer rating: 5 out of 5 stars
  • The New Testament?
Manipulating the Mouse Embryo: A Laboratory Manual
Andras Nagy
Manufacturer: Cold Spring Harbor Laboratory Press
ProductGroup: Book
Binding: Paperback

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Similar Items:
  1. Atlas of Mouse Development Atlas of Mouse Development
  2. Mouse Phenotypes: A Handbook of Mutation Analysis Mouse Phenotypes: A Handbook of Mutation Analysis
  3. The Laboratory Mouse: Handbook of Experimental Animals (HANDBOOK OF EXPERIMENTAL ANIMALS) The Laboratory Mouse: Handbook of Experimental Animals (HANDBOOK OF EXPERIMENTAL ANIMALS)
  4. Molecular Cloning: A Laboratory Manual (3-Volume Set) Molecular Cloning: A Laboratory Manual (3-Volume Set)
  5. Mouse Development: Patterning, Morphogenesis, and Organogenesis Mouse Development: Patterning, Morphogenesis, and Organogenesis

ASIN: 0879695919

Book Description

Regarded as the "Bible" of mammalian embryo manipulation techniques since the 1986 publication of the first edition of Manipulating the Mouse Embryo: A Laboratory Manual, the third edition of this essential laboratory resource has now been completely reorganized, rewritten, and updated by a new cast of authors. The result is a compilation of new, cutting-edge protocols that include embryonic stem cell production and genetic manipulation, mouse chimeras, mouse cloning, assisted reproduction strategies (including intracytoplasmic sperm injection and in vitro fertilization), whole embryo culture systems, electroporation, embryo and gamete cryopreservation and rederivation, and gene expression, as well as more extensive background information on the use of these techniques. The "gold standard" techniques for applying recombinant DNA technology to investigations of mammalian embryonic development included in the first two editions of this book are also updated and recast, as is the summary of the current state of understanding of mouse development at the molecular level. This book is the premier authoritative and comprehensive source of technical and theoretical guidance for mouse developmental biologists and geneticists and is an essential resource for newcomers to these fields.

Customer Reviews:

5 out of 5 stars The New Testament?.......2004-01-20

A definitive summary of mouse development and simple, concise proven protocols. This is the best publication there is for this kind of work. It's much better than the 2nd, ed. I wish it had more of a troubleshooting focus, but I guess if you are in this line of work, you should be able to figure out problems yourself, or query the tg list.... I like how there are alternative strategies mentioned depending on how your lab is equiped (or not).
Culture of Animal Cells: A Manual of Basic Technique
Average customer rating: 5 out of 5 stars
  • Fantastic book!
  • useful book
  • Save on Textbook
  • Best Basic Book on Tisssue Culture
  • The best book on cell culture
Culture of Animal Cells: A Manual of Basic Technique
R. Ian Freshney
Manufacturer: John Wiley & Sons
ProductGroup: Book
Binding: Hardcover

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  5. Short Protocols in Molecular Biology (2 volume set) Short Protocols in Molecular Biology (2 volume set)

ASIN: 0471453293

Book Description

The most complete resource on the techniques, equipment,principles, and practices of animal cell culture

Since publication of the previous edition of this benchmark text, numerous groundbreaking advances have occurred in stem cell research, cloning, tissue engineering, and in vitro toxicity testing. These and other developments have been incorporated into this fully revised and expanded Fifth Edition of Culture of Animal Cells. In addition, to answer the needs of the exponential increase in newcomers to cell culture, particularly in the biopharmaceutical industry, a completely new chapter on training in cell culture technology has been introduced.

The most complete resource on the techniques, equipment, principles, and practices of animal cell culture, this text offers a complete background related to growth of animal cells in culture. Beginning with laboratory design, safety, validation and bioethics, then continuing with preparation of media, primary culture and cell lines, through to characterization and authentication, contamination, specialized techniques, and troubleshooting, the coverage includes:
* An all-new section of training exercises, separated into basic, intermediate, and advanced procedures, cross-referenced to the relevant protocols
* New coverage of stem cells, bioethics, validation, cloning, cell signaling, in vitro toxicity testing, and tissue engineering
* An expanded full-color atlas section, with images of primary culture, cell lines, subculture, differentiation, cancer cells and transformation, three-dimensional culture, contamination, and specialized equipment
* Enhanced treatment of troubleshooting, with full cross-referencing to the relevant protocols and sections of text
* Fully updated references
* The clearest, most consistent presentation of step-by-step protocols available
* Numerous diagrams, photographs, tables, and charts
* Detailed and up-to-date information on reagent preparation and sourcing of materials and equipment, including a fully updated list of suppliers and other resources with Web sites

Indispensable for clinical and biopharmaceutical researchers and scientists, students, trainees, and technicians, this landmark text presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells.

Customer Reviews:

5 out of 5 stars Fantastic book!.......2007-09-08

Based on protocols in this book, I devised methods to complete my own experiments. I found the insights contained in the book completely indispensable. The range of topics it contains and the detail with which Freshney explains each point made the process of designing conditions for experiments rational and approachable. I highly recommend this book.

5 out of 5 stars useful book.......2007-08-09

There are not a lot of glossy pretty pictures, which is a good thing for a no-nonsense instruction manual that gets the point across with what pictures it did have. I found it to be a very good complement to my cell culture class. Everything I learned in the lab was put into context nicely.

5 out of 5 stars Save on Textbook.......2006-08-15

I purchased this book with a description of like new. I would say this is new. The previous person who used this did not even break in the binder. This is an excellent reference book, I have enjoyed reading the text up to this point.

5 out of 5 stars Best Basic Book on Tisssue Culture.......2006-07-07

For many years Freshney's Culture of Animal Cells has been the gold standard of tissue culture reference books. This new edition adds and updates many techniques and continues to be the best book in its area. I use it extensively both for my own research and in teaching classes on tissue culture.

5 out of 5 stars The best book on cell culture.......2005-01-09

Freshney's Culture of Animal Cells is a great book. It is writen on an easy style, it is concise and complete at the same time and it does teach you all the basics (and a little bit more) of cell culture. When I was doing my masters I didn't have a lot of help with my cell culture experiments and this book was fundamental for my research. The book covers everything, starting from the biology of cultured cells, equipments, design of the lab and asseptic technique and going all the way to primary culture, cloning and selection, cell separation, characterization, differentiation and more. The book (hard cover) is pretty resistant and it easily stands going to the bench (where you surely will be using it a lot). If you are thinking about spending money on a book about cell culture techniques this is the book! Don't think twice.
Pcr Primer: A Laboratory Manual
Average customer rating: 5 out of 5 stars
  • Best PCR manual there is!
Pcr Primer: A Laboratory Manual

Manufacturer: Cold Spring Harbor Laboratory Press
ProductGroup: Book
Binding: Hardcover

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Similar Items:
  1. PCR (The Basics) PCR (The Basics)
  2. Real-time PCR (BIOS Advanced Methods) Real-time PCR (BIOS Advanced Methods)
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  4. Lab Math: A Handbook of Measurements, Calculations, and Other Quantitative Skills for Use at the Bench Lab Math: A Handbook of Measurements, Calculations, and Other Quantitative Skills for Use at the Bench
  5. Molecular Cloning: A Laboratory Manual (3-Volume Set) Molecular Cloning: A Laboratory Manual (3-Volume Set)

ASIN: 0879696532

Book Description

From its first-published account in 1985, the polymerase chain reaction has become a standard research tool in a wide range of laboratories. Its impact has been felt in basic molecular biological research, clinical research, forensics, evolutionary studies, and the Human Genome Project. The PCR technique originally conceived by Nobel laureate Kary Mullis has proven to be exceptionally adaptable and has been transformed into a myriad array of methods, each with different applications.

PCR Primer: A Laboratory Manual introduces the complex world of PCR by beginning at an accessible level and then moving to more advanced levels of application. First, the practical requirements for performing PCR and other amplification techniques in the lab are introduced and then the basic aspects of the technique are explained by exploring important issues such as sample preparation, primer design, efficiency, detection of products, and quantitation. Protocols for a wide range of PCR and amplification techniques—each written by an expert investigator—are presented for cloning, sequencing, mutagenesis, footprinting, library construction and screening, exon trapping, differential display, and expression, and these include RT-PCR, RNA PCR, LCR, multiplex PCR, panhandle PCR, capture PCR, expression PCR, 3' and 5' RACE, immune PCR, in situ PCR, and ligation-mediated PCR. Each protocol is augmented by analysis and troubleshooting sections and complete references.

Customer Reviews:

5 out of 5 stars Best PCR manual there is!.......2000-09-20

I have used two different protocols from this book, 1, RACE-PCR and 2, mutagenesis.These two methods were described very well, both theoretically and practically. The protocols were described in detail, were easy to follow, and they work! I'm sure that the other protocols in this book also work equally well. If you do PCR, you need this book!
DNA Science: A First Course, Second Edition
Average customer rating: 5 out of 5 stars
  • My High School Textbook
  • Not Bad
  • Great Intro to Genetic Science
  • spotless
DNA Science: A First Course, Second Edition
David A. Micklos
Manufacturer: Cold Springs Harbor Laboratory Press
ProductGroup: Book
Binding: Hardcover

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  1. Molecular Biotechnology: Principles and Applications of Recombinant DNA Molecular Biotechnology: Principles and Applications of Recombinant DNA
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  5. Molecular Biology of the Gene, Fifth Edition Molecular Biology of the Gene, Fifth Edition

ASIN: 0879696362

Book Description

This is the second edition of a highly successful textbook (over 50,000 copies sold) in which a highly illustrated, narrative text is combined with easy-to-use thoroughly reliable laboratory protocols. It contains a fully up-to-date collection of 12 rigorously tested and reliable lab experiments in molecular biology, developed at the internationally renowned Dolan DNA Learning Center of Cold Spring Harbor Laboratory, which culminate in the construction and cloning of a recombinant DNA molecule.

Proven through more than 10 years' of teaching at research and nonresearch colleges and universities, junior colleges, community colleges, and advanced biology programs in high school, this book has been successfully integrated into introductory biology, general biology, genetics, microbiology, cell biology, molecular genetics, and molecular biology courses.

The first eight chapters have been completely revised, extensively rewritten, and updated. The new coverage extends to the completion of the draft sequence of the human genome and the enormous impact these and other sequence data are having on medicine, research, and our view of human evolution. All sections on the concepts and techniques of molecular biology have been updated to reflect the current state of laboratory research.

The laboratory experiments cover basic techniques of gene isolation and analysis, honed by over 10 years of classroom use to be thoroughly reliable, even in the hands of teachers and students with no prior experience. Extensive prelab notes at the beginning of each experiment explain how to schedule and prepare, while flow charts and icons make the protocols easy to follow.

As in the first edition of this book, the laboratory course is completely supported by quality-assured products from the Carolina Biological Supply Company, from bulk reagents, to useable reagent systems, to single-use kits, thus satisfying a broad range of teaching applications.

Customer Reviews:

5 out of 5 stars My High School Textbook.......2007-07-04

This was used for my high school semester course "DNA Sci part 1" textbook used with Watson's Recombinant DNA textbook. DNA Science:A 1st Course, 2nd edi was useful in supplementing the updates in recent genetic advances and some newer, better graphic representations. I found this to be an easy textbook to read, although not intended for those without a biology and chemistry background since the book does not waste much ink on describing what should have already been learned. The other students in my class seemed to find this book to be a clear, understandable textbook as well.

A very good first course textbook that keeps a student (or any person) interested. Nice big, clear print as well. The laboratories were the standard same as in the Recombinant DNA textbook.

4 out of 5 stars Not Bad.......2005-08-03

I havent read much of this yet, we really wont be using much until the second quarter but so far it seems not bad i hope this review is as helpful to you as it would have been for me oh yeah dna is interesting

5 out of 5 stars Great Intro to Genetic Science.......2005-07-28

This is the best single source of information on Genetic Science I have run across. Comprehensive from history, to theory, to lab practice. Highly recommended! Great illustrations and very clearly written. Its targeted to people with a serious interest in the field, definitely not for the "Genetic Science for Dummies" crowd.

5 out of 5 stars spotless.......2004-03-21

This book is the best book on the subject on the market. The subject matter is juxtaposed by easy yet informative experiments that can be carried out in a normal scholastic setting. I hear the writers are all hotties.
Molecular Cloning: A Laboratory Manual (3-Volume Set)
Average customer rating: 4.5 out of 5 stars
  • Molecular Cloning: A Laboratory Manual (3-Volume Set)
  • a formerly essential classic
  • the BIBLE of every biologist
  • the BIBLE of every biologist
  • Excellent reference for all
Molecular Cloning: A Laboratory Manual (3-Volume Set)
Joe Sambrook
Manufacturer: COLD SPRING HARBOR LABORATORY PRESS
ProductGroup: Book
Binding: Hardcover

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  1. Short Protocols in Molecular Biology (2 volume set) Short Protocols in Molecular Biology (2 volume set)
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  3. Lab Math: A Handbook of Measurements, Calculations, and Other Quantitative Skills for Use at the Bench Lab Math: A Handbook of Measurements, Calculations, and Other Quantitative Skills for Use at the Bench
  4. Culture of Animal Cells: A Manual of Basic Technique Culture of Animal Cells: A Manual of Basic Technique
  5. Lab Ref: A Handbook of Recipes, Reagents, and Other Reference Tools for Use at the Bench Lab Ref: A Handbook of Recipes, Reagents, and Other Reference Tools for Use at the Bench

ASIN: 0879695765

Book Description

The first two editions of this manual have been mainstays of molecular biology for nearly twenty years, with an unrivalled reputation for reliability, accuracy, and clarity.

In this new edition, authors Joe Sambrook and David Russell have completely updated the book, revising every protocol and adding a mass of new material, to broaden its scope and maintain its unbeatable value for studies in genetics, molecular cell biology, developmental biology, microbiology, neuroscience, and immunology.

Handsomely redesigned and presented in new bindings of proven durability, this three-volume work is essential for everyone using today's biomolecular techniques.

The opening chapters describe essential techniques, some well-established, some new, that are used every day in the best laboratories for isolating, analyzing and cloning DNA molecules, both large and small.

These are followed by chapters on cDNA cloning and exon trapping, amplification of DNA, generation and use of nucleic acid probes, mutagenesis, and DNA sequencing.

The concluding chapters deal with methods to screen expression libraries, express cloned genes in both prokaryotes and eukaryotic cells, analyze transcripts and proteins, and detect protein-protein interactions.

The Appendix is a compendium of reagents, vectors, media, technical suppliers, kits, electronic resources and other essential information.

As in earlier editions, this is the only manual that explains how to achieve success in cloning and provides a wealth of information about why techniques work, how they were first developed, and how they have evolved.

Customer Reviews:

5 out of 5 stars Molecular Cloning: A Laboratory Manual (3-Volume Set).......2007-03-19

This revised version of the Standard Lab Handbook has been improved, completed with new techniques and search is facilitated by the indices added at the end of each volume.

3 out of 5 stars a formerly essential classic.......2005-11-24

For many years the previous edition of this set was an essential reference in molecular biology labs. At present however, there are too many good protocol books out there to really make this argument. The book is pretty strong in explaining theory, and answering the question of why certain procedures are either necessary, useful, or worthless, however it is not as practical as many other books, such as Short Protocols. Still a good reference overall, but no longer stands alone, and I recommend checking out as much of the competition as possible before deciding whether to make the investment in it.

5 out of 5 stars the BIBLE of every biologist.......2003-01-31

So few and so much to say about this bible of Biology at the bench...
You'll really find everything you want in it, including the composition of all the buffers and solutions, the new protocols for high-tech biology (FLIM-FRET), some paragraphs about bioinformatics and more.Incredibly precise, this book is consequently a big book (3 huge volumes), so better know exactly wath you're looking for before opening it!
The must have of every lab!

5 out of 5 stars the BIBLE of every biologist.......2003-01-31

So few and so much to say about this bible of Biology at the bench...
You'll really find everything you want in it, including the composition of all the buffers and solutions, the new protocols for high-tech biology (FLIM-FRET), some paragraphs about bioinformatics and more.Incredibly precise, this book is consequently a big book (3 huge volumes), so better know exactly wath you're looking for before opening it!
The must have of every lab!

5 out of 5 stars Excellent reference for all.......2002-03-09

In this 3 volume set of books the authors summarize the most important laboratory protocols for DNA analysis and cloning. As someone involved in computational biology and mathematical gene sequence analysis, I was needing such a summary to get an idea of just how genetic engineering is actually practiced in the laboratory. The book is definitely written for those readers that are very experienced in these "wet" techniques, but it still could be perused profitably by anyone who is curious about genetic engineering. There is also an excellent website that owners of the books can go to and search for protocols and obtain updates and additions to the protocols.

At the beginning of each chapter, the authors give an introduction to the protocols and this is of an enormous help to those readers with only rudimentary acquaintance with the laboratory procedures. Typically, this introduction contains an historical summary of the procedures as they were developed or discovered. One can only marvel at the ingenuity of the discoverers of these techniques. These introductions are fairly straightforward to read, even for those that are not experts in biochemistry.

At the end of each chapter, the authors include an "information panel" that gives a more in-depth view of the biochemistry or genetics behind the procedures. These are summaries and are highly specialized, and are again meant for experienced readers. A very lengthy list of references is also included at the end of each chapter.

Becuase of the size of this collection, space here does not permit a detailed review, so I will list some of the areas that I thought were particularly interesting or well-written (these coming from the introduction or the information panels only): 1. The DNA synthesis at the colE1 replicon and the interaction between RNAI and RNAII. 2. The discussion of electroporation and the physics behind this technique to introduce DNA into eukaryotic cells. 3. The discussion on the discovery of bacteriophage lambda. 4. The discussion (with diagram), of the assembly pathway of bacteriophage lambda. 5. The summary of the early analysis of DNA using electrophoresis and the different pulsed-field configurations used. 6. The anecdote on the discovery of the polymerase chain reaction. 7. The short discussion on computer-assisted design of oligonucleotide primers. 8. The discussion of oligonucleotide synthesis. 9. The flowchart detailing the preparing and screening of a cDNA library. 10. The history of the development of the methods to synthesize and clone cDNAs. 11. The detailed discussion of the molecular cloning of double-stranded cDNA. 12. The discussion on the methods to validate clones of cDNA. 13. The discussion on magnetic beads for affinity purification. 14. The discussion on the history of DNA sequencing and the different techniques to accomplish it, particularly the information panel on automated DNA sequencing. 15. The discussion of the different types of mutagenesis and the different methods for accomplishing it. 18. The fascinating discussion of how to introduce cloned genes into mammalian cells. 19. The discussion on the steps involved in DNA footprinting. 20 The discussion on green flourescent protein and its use as a fusion tag. 21. The discussion on the use of surface plasmon resonance spectroscopy.
Drosophila Protocols
Average customer rating: 4.5 out of 5 stars
  • Comprehensive in scope, not quite enough detail.
  • Comprehensive in scope, not quite enough detail.
  • A must have for any Drosophila lab
Drosophila Protocols
William Sullivan
Manufacturer: COLD SPRING HARBOR LABORATORY PRESS
ProductGroup: Book
Binding: Hardcover

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  1. Drosophila: A Laboratory Handbook Drosophila: A Laboratory Handbook
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  5. Molecular Cloning: A Laboratory Manual (3-Volume Set) Molecular Cloning: A Laboratory Manual (3-Volume Set)

ASIN: 0879695862

Book Description

This exceptional laboratory manual describes thirty-seven procedures most likely to be used in the next decade for molecular, biochemical, and cellular studies on Drosophila. They were selected after extensive consultation with the research community and rigorously edited for clarity, uniformity, and conciseness.

The outstanding features of this protocol collection are:

- Scope: The methods included permit investigation of chromosomes, cell biology, molecular biology, genomes, biochemistry, and development

- Depth: Each protocol includes the basic information needed by novices, with sufficient detail to be valuable to experienced investigators.

- Format: Each method is carefully introduced and illustrated with figures, tables, illustrations, and examples of the data obtainable.

- Added value: The book's appendices include key aspects of Drosophila biology, essential solutions, buffers, and recipes.

An evolution of Michael Ashburner's 1989 classic Drosophila: A Laboratory Manual, this book is an essential addition to the personal library of Drosophila investigators and an incomparable resource for other research groups with goals likely to require fly-based technical approaches.

Customer Reviews:

4 out of 5 stars Comprehensive in scope, not quite enough detail........2000-06-23

This book is very impressive in scope, and (as other reviewers have said) IS indispensable for any Drosophila laboratory. It is a very valuable addition to every Drosophila laboratory. The book covers virtually every area of Drosophila work. Many of the topics discussed are cutting edge, such as the chapter on RNA interference, targeted cell ablation, and biochemical preparations from Drosophila (such as soluble nuclear extracts and membrane proteins). Overall I am very pleased with the book, and glad I bought it! One section of the book I find particularly useful is the beginners guide to the Drosophila genome project. This section overviews the information available from the recently completed Drosophila genome project. It tell us what kinds of information are available about the fly genome, how this information was collected, and where we can download it from the internet (complete with internet web site addresses)!

My only criticism of the book is a criticism which applies to ALL manuals published by the Cold Spring Harbor Laboratory Press: the protocols are too superficial, and not dealt with in sufficient detail to allow a novice to carry out these techniques (which is what a manual ideally SHOULD do). This has been true of all the CSHLP manuals I have seen. Conspicuously absent from the CSHL publications is a TROUBLESHOOTING section, which can be referred to if the researcher runs into problems. As we all know, there are many small (and seemingly trivial) details to any protocol which, if not carried out properly, can make or break an experiment. Some steps in a protocol are always more fastidious than others, and it is critical for us to know which steps can be done with a bucket and which steps require an eyedropper.

For my money, the best and most comprehensive manual is the CURRENT PROTOCOLS IN MOLECULAR BIOLOGY manual (Wiley and Sons), which is updated regularly, and has extensive troubleshooting sections attached to each protocol. (They have recently come out with a companion manual dealing exclusively with protein techniques.) Also, I highly recommend the METHODS IN MOLECULAR BIOLOGY series (Humana Press) for its concise protocols and supplementary troubleshooting sections. If the researcher is familiar with the techniques the troubleshooting sections need not be consulted, but if the researcher is a novice the troubleshooting sections are invaluable. Unfortunately a Drosophila manual is not available from either of these publishers, but this CSHL manual makes a good substitute. I recommend this book for all Drosophilists!

4 out of 5 stars Comprehensive in scope, not quite enough detail........2000-06-23

This book is very impressive in scope, and (as other reviewers have said) IS indispensable for any Drosophila laboratory. It is a very valuable addition to every Drosophila laboratory. The book covers virtually every area of Drosophila work. Many of the topics discussed are cutting edge, such as the chapter on RNA interference, targeted cell ablation, and biochemical preparations from Drosophila (such as soluble nuclear extracts and membrane proteins). Overall I am very pleased with the book, and glad I bought it! One section of the book I find particularly useful is the beginners guide to the Drosophila genome project. This section overviews the information available from the recently completed Drosophila genome project. It tell us what kinds of information are available about the fly genome, how this information was collected, and where we can download it from the internet (complete with internet web site addresses)!

My only criticism of the book is a criticism which applies to ALL manuals published by the Cold Spring Harbor Laboratory Press: the protocols are too superficial, and not dealt with in sufficient detail to allow a novice to carry out these techniques (which is what a manual ideally SHOULD do). This has been true of all the CSHLP manuals I have seen. Conspicuously absent from the CSHL publications is a TROUBLESHOOTING section, which can be referred to if the researcher runs into problems. As we all know, there are many small (and seemingly trivial) details to any protocol which, if not carried out properly, can make or break an experiment. Some steps in a protocol are always more fastidious than others, and it is critical for us to know which steps can be done with a bucket and which steps require an eyedropper.

For my money, the best and most comprehensive manual is the CURRENT PROTOCOLS IN MOLECULAR BIOLOGY manual (Wiley and Sons), which is updated regularly, and has extensive troubleshooting sections attached to each protocol. (They have recently come out with a companion manual dealing exclusively with protein techniques.) Also, I highly recommend the METHODS IN MOLECULAR BIOLOGY series (Humana Press) for its concise protocols and supplementary troubleshooting sections. If the researcher is familiar with the techniques the troubleshooting sections need not be consulted, but if the researcher is a novice the troubleshooting sections are invaluable. Unfortunately a Drosophila manual is not available from either of these publishers, but this CSHL manual makes a good substitute. I recommend this book for all Drosophilists!

The complete index of DROSOPHILA PROTOCOLS (CSHLP) is available at the publishers website given below...

5 out of 5 stars A must have for any Drosophila lab.......2000-04-20

This latest edition by Sullivan et al. is the best protocols manual for Drosophila that I've seen. It should be a staple for any fly lab. It provides the most up-to-date protocols for common techniques (ie staining, dissections, cytology) as well as more recent additions (i.e. DNA microarrays, neuroactive drugs, GFP reporters). Moreover, the protocols are clear, detailed, and easy to follow.
Basic Molecular Biology: Essential Techniques
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    Basic Molecular Biology: Essential Techniques
    Philippa D. Darbre
    Manufacturer: John Wiley & Sons
    ProductGroup: Book
    Binding: Spiral-bound

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    ASIN: 0471977055

    Book Description

    For the increasing number of professionals needing to learn about and use molecular biology techniques in their research, the daunting challenge is to break through the technical and largely inaccessible jargon found in most texts. This unique introduction to the field makes things much easier. The author begins with a brief overview of the basics, then focuses on simple step-by-step protocols of the core techniques, giving a theoretical outline, valuable advice on real-world problems readers might encounter, and estimates on how much time is required to perform each technique.
    Adenovirus Methods and Protocols, Vol 2: Ad Proteins, RNA, Lifecycle, Host Interactions, and Phyologenetics (Methods in Molecular Medicine)
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      Adenovirus Methods and Protocols, Vol 2: Ad Proteins, RNA, Lifecycle, Host Interactions, and Phyologenetics (Methods in Molecular Medicine)
      William S.M., Ed. Wold
      Manufacturer: Humana Press
      ProductGroup: Book
      Binding: Hardcover

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      ASIN: 1588299015

      Book Description

      Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. In addition to updating and expanding important chapters from the first edition, the authors have added new chapters that address innovative, exciting areas of emphasis in Ad research, including Ad vector construction and use, real-time PCR, use of new animal models, and methods for quantification of Ad virus or virus expression/interactions. Each of the protocols presented in these volumes is written by trendsetting researchers in their respective areas of expertise.

      Volume 1 addresses several important techniques for construction of adenoviruses for use as vectors and for basic research. Highlighted topics include deletion mutants, capsid modifications, insertions, and gene replacements in human, murine, bovine, and ovine adenoviruses. In Volume 2, the authors focus on methods that elucidate and quantitate the interactions of Ad with the host. Each of the protocols in these volumes provides a general introduction, followed by tried-and-true step-by-step methods. Both novice and experienced researchers will reap tremendous benefit from these groundbreaking volumes in Ad research.

      Glycobiology Protocols (Methods in Molecular Biology (Cloth))
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        Glycobiology Protocols (Methods in Molecular Biology (Cloth))
        Inka, Ed. Brockhausen
        Manufacturer: Humana Press
        ProductGroup: Book
        Binding: Hardcover

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        ASIN: 1588295532

        Book Description

        A distinguished panel of international experts reviews the importance of glycobiology, an interdisciplinary field encompassing chemistry, biochemistry, biology, physiology, and pathology. Glycobiology Protocols highlights important methodological progress that has been made in the field of glycobiology, helping scientists to answer specific questions on glycoprotein structures, on the biosynthesis of glycoconjugates, and on the functions of lipid- or protein-bound carbohydrates.
        Glycobiology Protocols contains specific methods for the analysis of the structures or functions of glycoconjugates, as well as of glycosyltransferases and glycosidases involved in the biosynthesis of glycans. The methods described for specific systems can be modified for investigations of similar biomolecules and tissues or cell types. Comprehensive and highly practical, Glycobiology Protocols provides researchers with a solid basis for studying the many aspects of glycobiology with state-of-the-art methods. The protocols follow the successful Methods in Molecular Biologyâ„¢ series format, with each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
        Mouse Genetics and Transgenics: A Practical Approach
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          Mouse Genetics and Transgenics: A Practical Approach
          I.J., Ed. Jackson
          Manufacturer: Oxford University Press
          ProductGroup: Book
          Binding: Hardcover

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          5. What's Wrong with My Mouse?: Behavioral Phenotyping of Trangenic and Knockout Mice What's Wrong with My Mouse?: Behavioral Phenotyping of Trangenic and Knockout Mice

          Accessories:
          1. Health o Meter  HDC100-01 "Grow with Me" Teddy Bear Scale for Babies and Toddlers Health o Meter HDC100-01 "Grow with Me" Teddy Bear Scale for Babies and Toddlers
          2. Braun IRT 4020 ThermoScan Ear Thermometer Braun IRT 4020 ThermoScan Ear Thermometer

          ASIN: 0199637083

          Book Description

          A unique book that integrates knowledge from a wide range of expertise, specifically applied to the mouse, and addressed at a wide audience from those new to the field to experts who want an update on the state of the art. Mouse Genetics and Transgenics: APA covers all aspects of using the mouse as a genetic model organism: care and husbandry; archiving stocks as frozen embryos or sperm; making new mutations by chemical mutagenesis; transgenesis; and gene targetting; mapping mutations and polygenic traits by cytogenetic, genetic, and physical means; and disseminating and researching information via the Internet.

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          10. Of Moths and Men: An Evolutionary Tale: The Untold Story of Science and the Peppered Moth

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