Condensed Protocols from Molecular Cloning: A Laboratory Manual

Book Description

The Condensed Protocols From Molecular Cloning: A Laboratory Manual is a single-volume adaptation of the three-volume third edition of Molecular Cloning: A Laboratory Manual. This condensed book contains only the step-by-step portions of the protocols, accompanied by selected appendices from the world's best-selling manual of molecular biology techniques. Each protocol is cross-referenced to the appropriate pages in the original manual. This affordable companion volume, designed for bench use, offers individual investigators the opportunity to have their own personal collection of short protocols from the essential Molecular Cloning

Customer Reviews:

Condensed protocols from Molecular Cloning: A laboratory manual.......2006-07-31

The condensed protocols version of Molecular Cloning is well written, concise and adequately referenced. Protocols are easy to follow and provide options depending upon individual experimental needs and preference.

The protocols provide information from home made recipes to prepared reagents available commercially.

The protocols work in my hands.

The book is large and not easily used on bench top. Otherwise it is a good resource for the laboratory.

Molecular Cloning: A Laboratory Manual (3-Volume Set)

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Molecular Cloning: A Laboratory Manual (3-Volume Set)
a formerly essential classic
the BIBLE of every biologist
the BIBLE of every biologist
Excellent reference for all

Molecular Cloning: A Laboratory Manual (3-Volume Set)
Joe Sambrook
Manufacturer: COLD SPRING HARBOR LABORATORY PRESS
ProductGroup: Book
Binding: Hardcover

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Short Protocols in Molecular Biology (2 volume set)
At the Bench: A Laboratory Navigator
Lab Math: A Handbook of Measurements, Calculations, and Other Quantitative Skills for Use at the Bench
Culture of Animal Cells: A Manual of Basic Technique
Lab Ref: A Handbook of Recipes, Reagents, and Other Reference Tools for Use at the Bench

ASIN: 0879695765

Book Description

The first two editions of this manual have been mainstays of molecular biology for nearly twenty years, with an unrivalled reputation for reliability, accuracy, and clarity.

In this new edition, authors Joe Sambrook and David Russell have completely updated the book, revising every protocol and adding a mass of new material, to broaden its scope and maintain its unbeatable value for studies in genetics, molecular cell biology, developmental biology, microbiology, neuroscience, and immunology.

Handsomely redesigned and presented in new bindings of proven durability, this three-volume work is essential for everyone using today's biomolecular techniques.

The opening chapters describe essential techniques, some well-established, some new, that are used every day in the best laboratories for isolating, analyzing and cloning DNA molecules, both large and small.

These are followed by chapters on cDNA cloning and exon trapping, amplification of DNA, generation and use of nucleic acid probes, mutagenesis, and DNA sequencing.

The concluding chapters deal with methods to screen expression libraries, express cloned genes in both prokaryotes and eukaryotic cells, analyze transcripts and proteins, and detect protein-protein interactions.

The Appendix is a compendium of reagents, vectors, media, technical suppliers, kits, electronic resources and other essential information.

As in earlier editions, this is the only manual that explains how to achieve success in cloning and provides a wealth of information about why techniques work, how they were first developed, and how they have evolved.

Customer Reviews:

Molecular Cloning: A Laboratory Manual (3-Volume Set).......2007-03-19

This revised version of the Standard Lab Handbook has been improved, completed with new techniques and search is facilitated by the indices added at the end of each volume.

a formerly essential classic.......2005-11-24

For many years the previous edition of this set was an essential reference in molecular biology labs. At present however, there are too many good protocol books out there to really make this argument. The book is pretty strong in explaining theory, and answering the question of why certain procedures are either necessary, useful, or worthless, however it is not as practical as many other books, such as Short Protocols. Still a good reference overall, but no longer stands alone, and I recommend checking out as much of the competition as possible before deciding whether to make the investment in it.

the BIBLE of every biologist.......2003-01-31

So few and so much to say about this bible of Biology at the bench...
You'll really find everything you want in it, including the composition of all the buffers and solutions, the new protocols for high-tech biology (FLIM-FRET), some paragraphs about bioinformatics and more.Incredibly precise, this book is consequently a big book (3 huge volumes), so better know exactly wath you're looking for before opening it!
The must have of every lab!

the BIBLE of every biologist.......2003-01-31

Excellent reference for all.......2002-03-09

In this 3 volume set of books the authors summarize the most important laboratory protocols for DNA analysis and cloning. As someone involved in computational biology and mathematical gene sequence analysis, I was needing such a summary to get an idea of just how genetic engineering is actually practiced in the laboratory. The book is definitely written for those readers that are very experienced in these "wet" techniques, but it still could be perused profitably by anyone who is curious about genetic engineering. There is also an excellent website that owners of the books can go to and search for protocols and obtain updates and additions to the protocols.

At the beginning of each chapter, the authors give an introduction to the protocols and this is of an enormous help to those readers with only rudimentary acquaintance with the laboratory procedures. Typically, this introduction contains an historical summary of the procedures as they were developed or discovered. One can only marvel at the ingenuity of the discoverers of these techniques. These introductions are fairly straightforward to read, even for those that are not experts in biochemistry.

At the end of each chapter, the authors include an "information panel" that gives a more in-depth view of the biochemistry or genetics behind the procedures. These are summaries and are highly specialized, and are again meant for experienced readers. A very lengthy list of references is also included at the end of each chapter.

Book Description

DNA microarray, or DNA chip, technology is a new and powerful means of discovering, characterizing, and analyzing genes and their expression patterns. The potential of this technology is huge and its applications are widespread across the many fields of biological and biomedical research. This manual assembles and synthesizes the expertise of over 30 innovators in this emerging field to provide authoritative, detailed instruction on the design, construction, and applications of microarrays, as well as comprehensive descriptions of the software tools and strategies required for extensive image and data analysis. Designed to extend and to complement the information in the best-selling laboratory manual Molecular Cloning, and presented in the same clear, user-friendly format, this book is an essential resource for investigators studying the molecular profiles of gene expression in any experimental system.

Customer Reviews:

Very useful.......2002-11-14

This book is very much like its well known predecessor, the currently 3 volume Molecular Cloning Manual with which it shares an editor and a similar title (in fact, it could pass as volume 4).
Overall it gives a good coverage of spotted microarray technology starting from the preparation of probes and slides to sample preparation and hybridization. In addition to expression profiling areas covered include uses of microarrays for analysis of chromatin immunoprecipitation samples, DNA copy number determination, and detection of genetic polymorphisms (oligo arrays). Tissue-arrays and micro-dissection techniques for sample preparation are also described. The bioinformatics section is less extensive than the experimental parts, but the chapters on clustering, self organizing maps and databases serve as good introductions to these areas. I especially liked the image acquisition, normalization and quality control sections. These reviews presented their material in a very clear and sensible way.
Each chapter starts with a short summary of the relevant background. These introductions are very concise, straightforward, jargon-free and up to date in their references. They are written by leading experts of their fields. In addition, there are several "Information Panels" with similar qualities, covering somewhat more general background material.
This is a very nice looking book; it is a pleasure to look at and to hold. I am sure every lab that uses microarrays could benefit from it.

Cloning, Gene Expression and Protein Purification: Experimental Procedures and Process Rationale

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Cloning, Gene Expression and Protein Purification: Experimental Procedures and Process Rationale
Charles et al. Hardin
Manufacturer: Oxford University Press
ProductGroup: Book
Binding: Paperback

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Molecular Biology

ASIN: 0195132947

Book Description

On the forefront of modern scientific innovation, Cloning, Gene Expression and Protein Purification: Experimental Procedures and Process Rationale effectively doubles as a laboratory manual for students and a reference book for professional researchers. Designed for advanced undergraduate and beginning graduate students in molecular biology, this unique combination lecture/laboratory resource presents detailed protocols for the multi-step process involved in isolating a gene, cloning and characterizing it, expressing its encoded protein, and purifying and characterizing the protein's basic physical properties. This manageable volume includes both theoretical background and practical procedures and is structured around twenty experiments that demonstrate how to prepare, manipulate, and analyze plasmids, produce fusion proteins in bacteria, and purify these proteins based on unique chemical properties or substrate affinities. The book describes advanced topics such as the use of antibodies and the techniques developed to transform their structures, as well as combinatorial approaches designed to manipulate the structure and functions of proteins and nucleic acids. Supplemental literature provides a variety of theoretical explanations encouraging a more intuitive understanding of the experimental mechanisms and behaviors of the chemical participants, while also giving students the tools needed to become "capable proactive researchers." Features: DT Emphasizes electrophoresis, Southern and Western blotting, and combinatorial techniques DT Defines clear reaction mechanisms; stipulates the functions of reagents; and helps students think about the precise consequences of solution and procedural manipulations DT Discusses fluorophores, and solvent effects on protein structure DT Characterizes plasmids, cDNAs, and antibody probes (available from ATCC) in research literature DT Includes carefully selected primary source research literature and articles from current vendor literature DT Contains a glossary of unfamiliar phrases and jargon; important summary statements and conclusions are italicized DT Provides an alphabetized list of common reagents for rapid reference DT Offers an extensive index of concepts and terms DT Categorizes helpful and distinctive information into five types of supplemental literature: Innovation/ Insight, Theory/Principle, Process Rationale, Vendor Literature, and Alternative Approaches

Protein-Protein Interactions: A Molecular Cloning Manual, Second Edition

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Revised, Updated, Expanded Second Edition
some good looking protocols - but bad mistakes: useless for a real scientist
simple mistakes - appears as no scientist ever checked for mistakes?

Protein-Protein Interactions: A Molecular Cloning Manual, Second Edition
Erica, Ed. Golemis
Manufacturer: Cold Spring Harbor Laboratory Press
ProductGroup: Book
Binding: Paperback

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Similar Items:

Molecular Cloning: A Laboratory Manual (3-Volume Set)
Protein-Protein Interactions: Methods and Protocols (Methods in Molecular Biology (Cloth))
Condensed Protocols from Molecular Cloning: A Laboratory Manual
Epigenetics
Lab Ref: A Handbook of Recipes, Reagents, and Other Reference Tools for Use at the Bench

ASIN: 0879697237
Release Date: 2005-10-31

Book Description

This is an updated edition of a manual that provides a thorough collection of the technical and theoretical issues involved in the study of protein associations, including standard methods, biophysical approaches, and, in a final section, a collection of computational methods for integrating and analyzing interactions.

Customer Reviews:

Revised, Updated, Expanded Second Edition.......2006-01-25

This second edition is much changed from the first edition of 2002. Most of the chapters in the earlier have been either extensively revised and brought up to date or replaced with new chapters (the old chapters are still available on a special web site).

The format of the book is the same with forty-six chapters written by the leading researchers in the field. The chapters are grouped into sections as follows:

1. Protein-Protein Interactions in Biological Context
2. Standard Technologies to Probe Protein Interactions
3. Biophysical Approaches to Probe Protein Interactions
4. Novel High-Throughput Approaches to Probe Protein Interactions
5. Interactions of Proteins and Peptides
6. InVivo Imaging of Protein Interactions
7. Genome-wide and Computer-based Analysis of Protein Interactions
8. Design of Novel Interactions and Inhibitors in Drug Discovery

This book is a summary of the state of the art in the field and is significantly improved over the first edition.

some good looking protocols - but bad mistakes: useless for a real scientist.......2005-09-27

Most of the protocols may be as good as they appear - no doubt about it! Most of the protocols have sure been written by well established scientists with a lot of input into details. But CSHLpress has an extremely good reputation to edit the very best books like the so-called "Maniatis" known for more than two decades as "the" standard in "molecular Cloning". With this respect the first edition of "Protein-protein interactions: A molecular cloning manual" only "copies" the famous word "cloning" on the title of the book and uses (legally) the logo "CSHLpress" to give the impression of a similar standard. But this impression is wrong: simple mistakes in the introduction of a method support strong doubts about how valuable the following protocol could be. This is not a good example of "CSHLpress"'s own extremely high standard. But hopefully most of the methods represent a good and detailed overview which may (or may not) work. I suggest to avoid the first edition and check carefully the 2nd edition soon. There is at least some room for improvement to reach the otherwise often unreached standards of CSHLpress.

simple mistakes - appears as no scientist ever checked for mistakes?.......2005-07-20

the very first impression of the book is pretty good: it appears to collect many useful and detailed protocols - useful especially for students and attractive for supervisors - and of course libraries. Unfortunately the first impression doesn't hold: very simple (and easily avoidable) mistakes show that neither the authors (or at least some of them) never ever checked for logistic mistakes - therefore it is very questionable if the protocols ever worked the way they were described. There is only little hope for an improvement of the (soon appearing) second editions. It may be worth for students to check the original literature where at least some peer review process could improve sophisticated protocols.

Bacterial Artificial Chromosomes Vol 1: Library Construction, Physical Mapping, and Sequencing (Methods in Molecular Biology (Cloth))

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Bacterial Artificial Chromosomes Vol 1: Library Construction, Physical Mapping, and Sequencing (Methods in Molecular Biology (Cloth))
Shaying, Ed. Zhao
Manufacturer: Humana Press
ProductGroup: Book
Binding: Hardcover

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Bacterial Artificial Chromosomes, Vol. 2: Functional Studies (Methods in Molecular Biology (Cloth))

ASIN: 0896039889

Book Description

For both volumes:

Expert investigators describe not only the classic methods, but also the many novel techniques they have perfected for the transfer of large DNAs into the cells of both microbes and animals via large-insert recombinant DNAs. Volume 1 presents readily reproducible techniques for library construction, physical mapping, and sequencing.. An accompanying volume, Volume 2: Functional Studies, provides a wide variety of methods and applications for functional analysis of the DNA-transformed organisms. Besides protocols, each chapter includes scientific reviews, software tools, database resources, genome sequencing strategies, and illustrative case studies.

Bacterial Artificial Chromosomes, Vol. 2: Functional Studies (Methods in Molecular Biology (Cloth))

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Bacterial Artificial Chromosomes, Vol. 2: Functional Studies (Methods in Molecular Biology (Cloth))
Shaying, Ed. Zhao
Manufacturer: Humana Press
ProductGroup: Book
Binding: Hardcover

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General | Science | Subjects | Books

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Bacterial Artificial Chromosomes Vol 1: Library Construction, Physical Mapping, and Sequencing (Methods in Molecular Biology (Cloth))

ASIN: 0896039897

Book Description

Basic Cloning Procedures (Springer Lab Manuals)

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Basic Cloning Procedures (Springer Lab Manuals)

Manufacturer: Springer
ProductGroup: Book
Binding: Paperback

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Book Description

This manual introduces the reader to basic methods used in the isolation, cloning and analysis of genetic material. The protocols include RT-PCR amplification, gene cloning, hybridization analysis and sequencing of nucleic acids, PCR-based site-specific mutagenesis, analysis of protein DNA-specific interaction, cell-free protein synthesis and product electrophoretic and immunological analysis. Each protocol includes short background information, a detailed description of the necessary materials, step-by-step procedures, a troubleshooting guide and useful practical hints.

Biotechnology: DNA to Protein -- A Laboratory Project in Molecular Biology

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Biotechnology: DNA to Protein -- A Laboratory Project in Molecular Biology
Teresa Thiel , Shirley T. Bissen , and Eilene M. Lyons
Manufacturer: McGraw-Hill Science/Engineering/Math
ProductGroup: Book
Binding: Spiral-bound

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Microbial Physiology and Metabolism
Recombinant DNA
Molecular Biology

ASIN: 0072416645

Book Description

This one-semester, project-based laboratory manual gives junior/senior level students the opportunity to characterize the enzyme alpha-amylase. As students proceed through the sequenced experiments, they will learn the principles of DNA, RNA, and protein structure by using modern-day laboratory techniques. Genetics, cell biology, and organic chemistry are prerequisites.

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