Transcriptional Regulation in Eukaryotes: Concepts, Strategies, and Techniques

Book Description

In the genome era, the analysis of gene expression has become a critical requirement in many laboratories. But there has been no comprehensive source of strategic, conceptual, and technical information to guide this often complex task. Transcriptional Regulation in Eukaryotes answers that need. Written by two experienced investigators, Michael Carey and Stephen Smale at the UCLA School of Medicine, and based in part on the Gene Expression course taught at Cold Spring Harbor Laboratory, this book directly addresses all the concerns of a laboratory studying the regulation of a newly isolated gene and the biochemistry of a new transcription factor.

Chapter 1 reviews the state-of-the-art in the RNA polymerase II transcription field;

Chapters 2-10 are a systematic guide to the investigation of regulatory mechanisms for newly identified genes;

Chapters 11-15 discuss approaches for synthesizing recombinant transcription factors, analyzing their binding, and deciphering their mode of action.

Transcriptional Regulation in Eukaryotes reveals not only what to do but why, and how to plan properly for success. This approach is ideal for graduate students, physician-scientists, postdocs, and others entering the field, but is also valuable for established investigators undertaking in-depth studies in specific systems.

Customer Reviews:

a great reference for transcription lab work!.......2005-02-18

I purchased this book for my lab and I cannot praise it highly enough. The only problem is that grad students and postdocs keep stealing it from my office and I'm always having to go out and hunt it down on someone's bench! When introducing a new technique to your lab, it is a good idea to read the chapater on that technique before you pick up your pipet. The descriptions of methodology and the degree of detail are first rate. I hope that Carey and Smale bring out a new edition soon that covers the new innovations in the transcription field from the five years since their current edition was published.

Lori Bernstein
Assistant Professor
Texas A & M University

Good Book for the beginner and expert...........2000-05-02

There are so many method for experiment regarding transcriptional regulations. In this book, you may find brief purpose, background knowledge and technical guide for the allmost experiment about transcriptional regulations.

Customer Reviews:

Excellent book, excellent!.......2006-03-29

I loved this book. It is so much more than a travel book. It was my guide when I first moved to Berkeley in 1996. It embodies so much about what Berkeley means, particularly the history about the Hillside club and the beautiful neighborhoods they left behind. Maybeck and crew saved the city and that wonderful hillside from being populated by little white boxes and made it something truly special. Anyway, if you are moving to Berkeley or live there already, this book will help you find the soul of the place.

Excellent,though dated alternative guide to Berkeley.......1997-11-07

An excellent, though dated (1989) guide to Berkeley. It covers restaurants, history, neighborhoods, and has several self guided tours. A must if you want to know much more about Berkeley.

Customer Reviews:

"Low lie the fields of Athenry".......2007-09-18

"By a lonely prison wall, I heard a young girl calling
'Michael, they have taken you away
For you stole Trevelyan's corn
So the young might see the morn'
Now a prison ship lies waiting in the bay..."

THE GREAT HUNGER is the definitive history of the Irish Potato Famine of 1845-1849. When the Englishwoman Cecil Woodham-Smith published this book in 1962 she was vilified and branded a Communist by the British establishment which had spent the previous 120 years explaining away what is undoubtedly the greatest European famine since antiquity. Estimates of the dead are difficult to quantify. Conservative historians put the number at 1-2 million; others place it closer to 6,000,000. At least another 1.5 million Irish fled their homeland.

Like most disasters, "An Gortha Mor" seems both inevitable and avoidable in retrospect. The Irish population exploded in the first half of the 19th century reaching an official 8.2 million (and an unofficial ten million) just before the Famine. But unlike Britain, which had become heavily industrialized and was moving confidently into the modern and scientific Victorian Era, Ireland was sunk in a morass of poverty and dejection. The average Irish countryman led a life no better than the poorest serfs of Imperial Russia of the day, and the Irish were subject to all manner of legal restrictions, mass unemployment, subsistence agriculture, exploitation by landlords, and eviction at whim from the land and their homes, often just a rude mud cabin. With no education, and few skills other than potato farming, eviction meant almost certain death for husbands, wives and children. Often, they were driven even from the bogs where they'd found shelter after being put out.

The Blight, too, meant certain death for far too many. Eating nothing but potatoes and buttermilk, these most wretched people literally had nothing at all to sustain them after the crop turned into a glutinous, stinking mass of black rot. They died in droves, particularly in the poor west of Ireland, bleak and rocky Connaught. The typhus which followed killed more.

As hideous as all this seems, Cecil Woodham-Smith tells us that the Blight was only one factor in the disaster that overtook the Irish. More insidious was the attitude of the British administration which largely stayed hardset in its laissez-faire attitude, refusing to step in and feed the Irish, refusing to interfere with the free market economy of the day, and worst of all, refusing to grasp that the market economy only works when people have money or skills to trade for products and services. In 1845, Ireland was still a pre-capitalist economy, and the mercantile approach of the British simply could not be applied there; still, the British tried, and blamed their own failure to address the Famine on their convenient perceptions of Irish intransigence and laziness.

Sir Charles Edward Trevelyan may be one of the most hated figures in Ireland even to this day. Effectively the head of British efforts at Famine Relief, Trevelyan was unenamored of the Irish, he was a rock-ribbed capitalist, and, though moral and moralistic to a fault, was also just as singleminded, blind to the suffering of the populace, but fixed on promoting Irish efforts at self-help. He bought a parsimonious 100,000 Pounds Sterling worth of unmilled American corn, and doled it out to provide for the eight million Irish. Amazingly, Trevelyan kept food EXPORTS flowing out of the country at pre-Famine levels throughout (!) Nothing could interfere with trade.

A disciple of the philosopher Thomas Malthus, Trevelyan cast a cold and dispassionate eye over Ireland's circumstances, seeing them as a form of natural population control. At the same time, the British placed the country under virtual martial law, decreeing "seven long years Transportation way on down to Van Diemen's Land" (Tasmania) for minor infractions and acts of desperation (such as stealing corn).

Was this, as many have posited, an organized genocide? Certainly, there were those among the British who despised the Irish to that extent. On the other hand, if this had been an organized killing field, then why did the British do anything at all to help the Irish, little as it was?

Woodham-Smith's tales of people living in bogs, of coffinless mass funerals, of fever patients being abandoned by their terrified relations, of Ireland starving to death, cannot help but touch the reader. The British are presented as less calculating than more stupid, unable to adjust their thought processes to meet the crisis. Conditions were so awful that when the Irish left Ireland (on rotten-bottomed Coffin Ships, like as not), their arrival in American and Canadian ports can be summed up shortly: NO IRISH NEED APPLY.

More than just a history of the Potato Famine, THE GREAT HUNGER is an indictment of the too-common human propensities of blaming the victim, making gestures instead of taking action, and that of ultimately doing nothing. The truth behind every human tragedy can be found in the pages of THE GREAT HUNGER.

This is an essential read.

A Masterpiece.......2007-05-24

I have read and reread this history several times and bought copies for
my sons.

I don't believe anyone can understand the Ireland of today without
this touching and tragic reference.

FACTUAL ACCOUNT OF THE FAMINE.......2006-03-16

In this account of the Famine,the author paints a picture of events which led up to ,and caused the Famine, the international poliics of the day, the weather patterns, the logistics of providing relief to so many destitute people.
Written factually and without blame it is a most interesting and informative read, I am glad I bought it.

Worthwhile Reminder.......2006-02-03

This history book reminds us that the Irish were mistreated in their homeland and in the USA when they first arrived. It tells of the heroic efforts to help the impoverished, illiterate populace and of the failed attempts by the British government to deal with a culture so foreign to their own.
It is a reminder of how far the Irish have come since the Celtic Tiger is rampant and people from Eastern Europe and the third world are going to Ireland for jobs and better lives.
Cecil Woodham-Smith is a British woman.

Had to read it for class.......2005-09-19

I had to read this novel for a college course on the British Empire. It is definitely not an easy read, but is extremely interesting if you can get through it (which I of course had to, to write a paper on it..). It is definitely one of the better assignments I have had to do.

Anyway, I just wanted to leave a comment, that I think its ridiculous that a handful of people that reviewed this book did not even realize that the book is written by a woman...

Average customer rating:

The Great Hunger: Ireland, 1845-1849
Cecil Blanche Fitzgerald Woodham-Smith
Manufacturer: Harper & Row
ProductGroup: Book
Binding: Hardcover
ASIN: 0241902177

Baking from the Heart: Our Nation's Best Bakers Share Cherished Recipes for The Great American Bake Sale (A Share Our Strength Book to Fight Hunger)

Average customer rating:

very unique - big variety
now fearless
A Wonderful Book!
Makes You Want to Bake!!
Great holiday gift - but buy one for yourself too!

Baking from the Heart: Our Nation's Best Bakers Share Cherished Recipes for The Great American Bake Sale (A Share Our Strength Book to Fight Hunger)
Michael J. Rosen
Manufacturer: Broadway
ProductGroup: Book
Binding: Hardcover

General | Baking | Cooking, Food & Wine | Subjects | Books

General | Cooking, Food & Wine | Subjects | Books
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ASIN: 0767916395

Book Description

Baking is about memories: recipes handed down from generation to generation and tastes that conjure childhood—think of Proust’s madeleines or your mom’s chocolate cake. Sweets are often bound up in our emotional life as adults, too: they’re how we reward ourselves or our children, how we celebrate holidays, birthdays, and special occasions, and how we honor guests.

In Baking from the Heart, more than fifty of the nation’s preeminent bakers share their recipes for cookies, cakes, and other dessert favorites, and the memories of why they hold that recipe dear. From the Apple Snacking Spice Cake that Joanna Chang made her fourth-grade teacher to show her how much she loved her to the Polvorones that were a Sunday after-church treat in Miguel Ravago’s home, these are recipes—and stories—to treasure.

When James Beard Award–winner Greg Patent was a teenager, he won a trip to New York City to compete in the Pillsbury Bake-Off with his Cherry-Apricot Coconut Bars. Forty years later, his mother earned a place in that same competition with her Walnut Fudge Bars. World-renowned chocolatier Jacques Torres tucked a few pints of hand-picked Michigan blueberries into his luggage so he could again make Blueberry Dame Blanche, the jam-filled cookies he made when he was a child in France, with his aging mother. For her son Gio’s first Valentine’s Day at school, Food TV’s Gale Gand concocted Marshmallow Heart Throbs, a cupcake he could cut into the shape of a heart. When Jimmy Schmidt’s family vacationed in Wisconsin, his contribution to his mother’s Black Walnut Pound Cake were the walnuts he picked and shelled with his siblings, aided by their father who would crack the hulls by driving over them in his ’55 Chevy. Like many of the other contributors, Jimmy Schmidt serves up two recipes with reminiscences (the walnut cake and his Blueberry Slump) for our delectation.

Baking from the Heart is also sweet inspiration for anyone who wants to join in The Great American Bake Sale™. When Share Our Strength—the nation’s preeminent hunger-fighting organization—joined with PARADE magazine to launch The Great American Bake Sale™ in 2003, the country’s response was overwhelming: nearly half a million people baked, bought, or sold, raising over a million dollars to end childhood hunger. (More information appears inside.)

A portion of the proceeds from the sale of this book benefit Share Our Strength, one of the nation’s preeminent anti-hunger agencies.

Customer Reviews:

very unique - big variety.......2005-12-23

I really enjoy this book. There is a cinnamon roll recipe in here that is to die for! The homemade oreos are wonderful and fun to make. The stories are interesting to read. There is many recipes in here that I would never make but when there is a book like this with such a variety of recipes, that is bound to happen. I collect baking cookbooks and one of the reasons that I purchased this one is because there is recipes in here that I have not seen in any other book. Very original.

now fearless.......2004-11-30

I'm not sure what I enjoyed the most - the photos, the recipes or the stories. Like so many people, I've had a love of cooking and fear of baking... until Baking From The Heart. While thumbing through my new copy, I stopped at Mohn Kickle, a poppy seed cookie I grew up eating but hadn't heard of or thought about in 20 years. As I read the introductory story by chef Lora Brody, I couldn't stop laughing as she shares hilarious stories about her parent's culinary skills. I decided to bake something that my 2 1/2 year old would enjoy and settled on David Lebovitz's peppermint patties. We loved them - as did everyone in my office the next day. I felt like such a big shot, making candy at virtually my first baking attempt. I hit a few bumps along the way but in the end, they looked and tasted delicious. Now I want to try literally every recipe in the book starting with the Flan Almendrado.

A Wonderful Book!.......2004-11-24

A beautiful book. Not your average cook book - the contributors to this book share with you where their recipe comes from - most often a family recipe passed down over time. A great gift (especially when paired with Cooking from the Heart) and a great opportunity to support Share Our Strength.

Makes You Want to Bake!!.......2004-11-03

Just in time for the holidays, this book has great recipes for those you love with sweet tooths. My personal favorites are the luscious peppermint patties and the homemade Oreos! Plus, I love the anecdotes by each chef. It makes each recipe much more personal. I love this book and I think you will too.

Great holiday gift - but buy one for yourself too!.......2004-11-02

I bought this book as a thank you gift for two friends who love to bake, but after reading through it, I decided I had to have one for myself. The Pumpkin Loaf Cake with Chocolate chips and the Chocolate Rasperry Chess Pie were both delicious and fairly easy to make. I have to say that I've never enjoyed reading a cookbook as much as this one. The stories are so much fun to read. If you like to bake, or want to start baking, you will really enjoy this collection - and knowing that proceeds will go to fight hunger makes it that much better.

James Joseph Sylvester: Life and Work in Letters

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James Joseph Sylvester: Life and Work in Letters
Karen Hunger Parshall
Manufacturer: Oxford University Press, USA
ProductGroup: Book
Binding: Hardcover

General | Biographies & Memoirs | Subjects | Books

Scientists | Professionals & Academics | Biographies & Memoirs | Subjects | Books

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All Titles | Qualifying Textbooks - Fall 2007 | Stores | Books
ASIN: 0198503911

Book Description

In the folklore of mathematics, James Joseph Sylvester (1814-1897) is the eccentric, hot-tempered, sword-cane-wielding, nineteenth-century British Jew who, together with the taciturn Arthur Cayley, developed a theory and language of invariants that then died spectacularly in the 1890s as a result of David Hilbert's groundbreaking, `modern' techniques. This, like all folklore, has some grounding in fact but owes much to fiction. The present volume brings together for the first time 140 letters from Sylvester's correspondence in an effort to establish the true picture. It reveals - through the letters as well as through the detailed mathematical and historical commentary accompanying them - Sylvester the friend, man of principle, mathematician, poet, professor, scientific activist, social observer, traveller. It also provides a detailed look at Sylvester's thoughts and thought processes as it shows him acting in both personal and professional spheres over the course of his eighty-two year life. The Sylvester who emerges from this analysis - unlike the Sylvester of the folkloric caricature - offers deep insight into the development of the technical and social structures of mathematics.

The Great Hunger and the Gallant John-Joe: The Gallant John-Joe

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The Great Hunger and the Gallant John-Joe: The Gallant John-Joe
Tom Mac Intyre
Manufacturer: Lilliput Press
ProductGroup: Book
Binding: Paperback

General | Performing Arts | Arts & Photography | Subjects | Books

General | Arts & Photography | Subjects | Books

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Feed the Children First: Irish Memories of the Great Hunger

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PART OF THE SERIES: ATHENEUM BOOKS FOR THE YOUNG READER, THIS STUDY IS IMPORTANT FOR ALL READERS OF EVERY AGE

Feed the Children First: Irish Memories of the Great Hunger

Manufacturer: Atheneum
ProductGroup: Book
Binding: Hardcover

Europe | History & Historical Fiction | Children's Books | Subjects | Books

General | Europe | History | Subjects | Books

Look Inside Teen Books | Trip | Specialty Stores | Books
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ASIN: 0689842260

Book Description

The great Irish potato famine -- the Great Hunger -- was one of the worst disasters of the nineteenth century. Within seven years of the onset of a fungus that wiped out Ireland's staple potato crop, more than a quarter of the country's eight million people had either starved to death, died of disease, or emigrated to other lands. Photographs have documented the horrors of other cataclysmic times in history -- slavery and the Holocaust -- but there are no known photographs whatsoever of the Great Hunger.

In Feed the Children First, Mary E. Lyons combines first-person accounts of those who remembered the Great Hunger with artwork that evokes the times and places and voices themselves. The result is a close-up look at incredible suffering, but also a celebration of joy the Irish took in stories and music and helping one another -- all factors that helped them endure.

Customer Reviews:

PART OF THE SERIES: ATHENEUM BOOKS FOR THE YOUNG READER, THIS STUDY IS IMPORTANT FOR ALL READERS OF EVERY AGE.......2007-01-30

Although this book is marketted for the middle school reader, its content and presentation makes it essential for everyone who wishes to understand the too unknown history of Ireland in the nineteenth century, in which Ireland due entirely to British imperialist policies lost four fifths of its population, as shown in this book. This book also relates the mass starvation of children to current juvenile malnutrition and death in African and other nations, and calls for us to act now to alleviate the suffering of the past. Websites are provided.

Obiovusly from this brief synopsis you can see the content of this book is quite substantial for the young reader, as necessary as it is that this history be made known. Genocidal British imperialist policies continue today, destroying children's lives in Fallujah and elsewhere in Iraq, with the US military as accomplices. Please read this book to understand the long history and roots of this imperial modus operandi, as this book also touches on the time of Henry VIII, of Cromwell and of the Penal Code which denied Irishmen education, land, language and religion, as the British demonized the Catholic Church as thoroughly as we now see Islam targeted, in order to rationalize its brutal genocidal policies, which led to the loss of four fifths of the Irish population.

As this book reveals, there was plenty of food in Ireland at the time of the so-called Famine, but it was all, every bit of corn, cattle, grains and other agricultural products taken to feed England under a severe sharecropping system which left Irishmen exiles and homeless and dispossessed in their own land, evicted from their own farms.

This book admirably uses primary sources and early illustrations to bring home the truth of this history. It is easily read but difficult to consider, and must be read by every Irish American, as well as everyone who cares enough to feed the starving chidlren of the world first.

Highly recommended readig for study and contemplation, and an excellent portal to further research at a more graduate academic level, of which books there are several here on the amazon.

Average customer rating:

Great Hunger: Ireland, 1845-49
Cecil Woodham-Smith
Manufacturer: New Eng. Lib.
ProductGroup: Book
Binding: Paperback
ASIN: 0450000133

Average customer rating:

The Great Hunger: Ireland, 1845-1849
Cecil Blanche Fitzgerald Woodham-Smith
Manufacturer: Harper & Row, New York
ProductGroup: Book
Binding: Hardcover
ASIN: B000I9QM14

Average customer rating:

Great Hunger: Ireland, 1845-1849
Cecil Blanche Fitzgerald Woodham-Smith
Manufacturer: Barnes Noble Books
ProductGroup: Book
Binding: Hardcover

General | Europe | History | Subjects | Books

Social History | Historical Study | History | Subjects | Books
ASIN: 0880293853

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