Book Description
The most complete resource on the techniques, equipment,principles, and practices of animal cell culture
Since publication of the previous edition of this benchmark text, numerous groundbreaking advances have occurred in stem cell research, cloning, tissue engineering, and in vitro toxicity testing. These and other developments have been incorporated into this fully revised and expanded Fifth Edition of Culture of Animal Cells. In addition, to answer the needs of the exponential increase in newcomers to cell culture, particularly in the biopharmaceutical industry, a completely new chapter on training in cell culture technology has been introduced.
The most complete resource on the techniques, equipment, principles, and practices of animal cell culture, this text offers a complete background related to growth of animal cells in culture. Beginning with laboratory design, safety, validation and bioethics, then continuing with preparation of media, primary culture and cell lines, through to characterization and authentication, contamination, specialized techniques, and troubleshooting, the coverage includes:
* An all-new section of training exercises, separated into basic, intermediate, and advanced procedures, cross-referenced to the relevant protocols
* New coverage of stem cells, bioethics, validation, cloning, cell signaling, in vitro toxicity testing, and tissue engineering
* An expanded full-color atlas section, with images of primary culture, cell lines, subculture, differentiation, cancer cells and transformation, three-dimensional culture, contamination, and specialized equipment
* Enhanced treatment of troubleshooting, with full cross-referencing to the relevant protocols and sections of text
* Fully updated references
* The clearest, most consistent presentation of step-by-step protocols available
* Numerous diagrams, photographs, tables, and charts
* Detailed and up-to-date information on reagent preparation and sourcing of materials and equipment, including a fully updated list of suppliers and other resources with Web sites
Indispensable for clinical and biopharmaceutical researchers and scientists, students, trainees, and technicians, this landmark text presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells.
Customer Reviews:
Fantastic book!.......2007-09-08
Based on protocols in this book, I devised methods to complete my own experiments. I found the insights contained in the book completely indispensable. The range of topics it contains and the detail with which Freshney explains each point made the process of designing conditions for experiments rational and approachable. I highly recommend this book.
useful book.......2007-08-09
There are not a lot of glossy pretty pictures, which is a good thing for a no-nonsense instruction manual that gets the point across with what pictures it did have. I found it to be a very good complement to my cell culture class. Everything I learned in the lab was put into context nicely.
Save on Textbook.......2006-08-15
I purchased this book with a description of like new. I would say this is new. The previous person who used this did not even break in the binder. This is an excellent reference book, I have enjoyed reading the text up to this point.
Best Basic Book on Tisssue Culture.......2006-07-07
For many years Freshney's Culture of Animal Cells has been the gold standard of tissue culture reference books. This new edition adds and updates many techniques and continues to be the best book in its area. I use it extensively both for my own research and in teaching classes on tissue culture.
The best book on cell culture.......2005-01-09
Freshney's Culture of Animal Cells is a great book. It is writen on an easy style, it is concise and complete at the same time and it does teach you all the basics (and a little bit more) of cell culture. When I was doing my masters I didn't have a lot of help with my cell culture experiments and this book was fundamental for my research. The book covers everything, starting from the biology of cultured cells, equipments, design of the lab and asseptic technique and going all the way to primary culture, cloning and selection, cell separation, characterization, differentiation and more. The book (hard cover) is pretty resistant and it easily stands going to the bench (where you surely will be using it a lot). If you are thinking about spending money on a book about cell culture techniques this is the book! Don't think twice.
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Hepatocyte Review
Michael N. Berry
Manufacturer: Kluwer Academic Publishers
ProductGroup: Book
Binding: Hardcover
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Atlas of Internal Medicine
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The Handbook of Contraception: A Guide for Practical Management (Current Clinical Practice)
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Principles of Molecular Medicine
ASIN: 0792361776 |
Book Description
The isolated hepatocyte preparation, employed in suspension or culture, is now firmly established as a major tool not only for the study of the liver but also for the investigation of a large number of aspects of biology common to many cell types.
The Hepatocyte Review provides a comprehensive description of recent advances in methodology and biology pertaining to the isolated hepatocyte preparation, and offers a unique insight into the value of isolated hepatocytes for basic and applied studies. A particular feature is the series of major reviews on growth regulation by hormones and growth factors, cell cycle progression and active cell death. The book also assembles for the first time novel work on the use of hepatocytes for clinical applications in the fields of transplantation, gene therapy and the bioartificial liver. Another important feature is a compilation of methods for isolated hepatocyte preparation from animals other than the rat, and includes several accounts of the isolation of human hepatocytes, which is becoming an increasingly important technique. A number of specialised techniques for hepatocyte manipulation, and a detailed description of new advances in hepatocyte culture, are also included.
The Hepatocyte Review is essential reading for any scientist using, or contemplating using, the isolated hepatocyte technique and will also be of major interest to cell biologists in general.
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Animal Cell Culture Methods (Methods in Cell Biology, Vol 57(Paper))
JENNIE P., ED. MATHER
Manufacturer: Academic Press
ProductGroup: Book
Binding: Spiral-bound
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ASIN: 0124800408 |
Book Description
This volume provides complete and thorough coverage of the classical and state-of-the-art methods used in cell culture. It also includes basic principles used in the selection of cells for specific scientific study, as well as analytical and procedural techniques.
Key Features
* Coverage of both classical and state-of-the-art methods used for cell culture
* Complete presentation of equipment and instrumentation options
* Guidelines for establishing, maintaining, and storing cell lines
* Information on sources for available cell lines
* Techniques for morphologic studies of cell cultures
* Reviews basic principles of cell culture
* Gives options and techniques on how to look at cells
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Animal Cell Biotechnology: Methods and Protocols (Methods in Biotechnology)
Manufacturer: Humana Press
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Binding: Hardcover
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ASIN: 0896035476 |
Book Description
Scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Capturing the major advances that have occurred in both the science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in genetically manipulating animal cells, optimizing their growth in defined media (particularly at large-scale), avoiding contamination, and in the harvesting and analysis of cell products. Topics include basic culture facilities and methods; molecular methods for gene transfection, cell immortalization and cell fusion; and techniques for the study of cell growth, viability, metabolism, and productivity. Animal Cell Biotechnology constitutes a comprehensive manual of state-of-the-art techniques for setting up a cell culture laboratory, maintaining cell lines, and optimizing critical parameters for cell culture.
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Large-scale Mammalian Cell Culture Technology (BIOPROCESS TECHNOLOGY)
LUBINIECKI
Manufacturer: CRC
ProductGroup: Book
Binding: Hardcover
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ASIN: 0824783271 |
Book Description
An interdisciplinary approach, integrating biochemistry, biology, genetics, and engineering for the effective production of protein pharmaceuticals. The volume offers a biological perspective of large-scale animal cell culture and examines diverse processing strategies, process management, regulator
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Techniques in Prion Research (METHODS AND TOOLS IN BIOSCIENCES AND MEDICINE)
Sylvain, Ed. Lehmann
Manufacturer: Springer-Verlag, Inc.
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ASIN: 3764322241 |
Book Description
Prion diseases, also known as Transmissible Spongiform Encephalopathies (TSEs), exist in both humans (Creutzfeldt-Jakob disease (CJD)) and animals (scrapie, bovine spongiform encephalopathy (BSE), chronic wasting disease) and have the unique property of being infectious, sporadic or genetic in origin.
Although the precise nature of the infectious agent responsible for TSEs is not definitely identified, it is now clearly demonstrated that a protein named PrP (for Prion Protein) plays a critical role in the transmission and pathogenesis of TSEs.
This book provides the general description as well as the details of the techniques currently used for the study of prion diseases. Taking into account the pivotal role played by PrP it is not surprising that many Chapters of this book deal with the purification, the detection and the characterization of the different forms of this protein. In addition, in vitro, cellular and animal models specifically adapted to the study of TSEs, as well as bio-safety procedures are described. Each Chapter is written by scientists involved for many years in their respective domain of prion biology who give the best of their knowledge in this technical document. This volume is a very useful tool for any laboratory which recently decided to contribute to the study of TSEs as well as for teams already engaged in this field for many years but interested in extending their technical capacity toward new methods.
Features:
Purification of PrPC and the Pathological Isoform of Prion Protein (PrPsc or PrP-res)
Animal Models of TSEs
Cell Culture Models of TSEs
PrPsc Immunohistochemistry
Western Immunoblotting Techniques
Antibody Production and ELISA
TSE Strain Typing in Mice
Biosafety and Decontamination Procedures
Cell-free Conversion of Prion Proteins
Cytotoxicity of PrP Peptides
Cyclic Amplification of Prion Protein Misfolding
Of interest to:
Researchers and clinicians in the fields of cell biology, biomedicine, neuroscience/neuropathology, veterinary medicine and biochemistry.
Book Description
Cell culture techniques are invaluable to the modern researcher but difficult to carry out successfully. As part of the series of Handbooks in Practical Animal Cell Biology, this volume offers a concise practical guide to the basic essentials of the technique. Researchers new to cell culture will find a clear explanation of the essential equipment of a tissue culture facility, including tissue culture media and sera. It describes methods for growing suspension and adhesion cultures, including how to store cells and prepare primary cultures from cells. For those already culturing cells, the handbook will act as a handy reference to the basic techniques. The essence of the book is to deal with the generalities of cell culture and to give a grasp of the basic concepts before involvement in more specialized work in the field. Ideal for anyone moving into tissue cell culture techniques or looking for a concise reference book.
Customer Reviews:
From Book to Bench-Cell Culturing Made Easy.......2000-05-16
Whether a first year college student or a seasoned researcher, General Techniques of Cell Culture is a must. GTCC simplifies the complicated world of cell culturing without insulting. The authors, Maureen A. Harrison and Ian F. Rae, make no assumptions about their readers, and take the task of explaining large amounts of scientific information in an organized and easy to read format. This handbook goes beyond general lab techniques and provides not only the "how-to", but also gives the developing lab the where at, as it rounds out each section with a directory of services and researchable references. By providing time honored techniques, General Techniques of Cell Culture also takes some of the trial and error out of propagating healthy cells in the lab. This alone should make the transition from book to bench less tedius and more productive.
Book Description
The Basics series provide the ideal introduction to new techniques in the laboratory. This series of books will allow you to use the techniques described, and to manipulate them to suit your own basic requirements.
This book describes the basic requirements for establishing and maintaining cell cultures both in the laboratory and in large-scale operations. Minimal background knowledge of the subject is assumed and therefore it will be a readable introduction to animal cell culture for scientists intending to initiate some work in this field.
New topics are included to reflect the latest developments and trends in the field. They cover the latest theory of the biological clock (teleomeres) of cell lines, the development of improved serum-free media formulations, the increased understanding of the importance and control of protein glycosylation and the humanization of antibodies for therapeutic use.
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Human Cell Culture: Primary Mesenchymal Cells (Human Cell Culture, Volume 5) (HUMAN CELL CULTURE)
Manfred R., Ed. Koller
Manufacturer: Kluwer Academic Publishers
ProductGroup: Book
Binding: Hardcover
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ASIN: 0792367618 |
Book Description
This unique comprehensive volume in the
Human Cell Culture Series encompasses research methodology for the growth and differentiation of all types of primary mesenchymal cells. Each of eleven mesenchymal cell types is covered by a leading expert in the field, providing insightful background information along with detailed current culture and assay techniques. Tissue types covered include bone, cartilage, tendons and ligaments, muscle (smooth, skeletal, and cardiac), adipose tissue, and skin (dermis), as well as multi-potent mesenchymal stem cells and fibrocytes. In addition to uses for research applications, current and future clinical applications of large-scale culture methods are also discussed. Further, the procurement and processing of primary human tissues is covered in detail. The generous inclusion of figures and tables, along with a consistent format, makes critical information highly accessible to the reader. Additionally, numerous literature citations provide a valuable reference for students and professionals in the fields of cell biology and bioengineering.
Book Description
Animal Cell Biotechnology: Methods and Protocols, Second Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture considering the whole cascade from lab to final production. The volume is divided into five parts that reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially transduction techniques, cells for gene therapy and antibody production. Part II addresses basic cultivation techniques, such as microcarrier culture and encapsulation.
Part III covers cell characterization and analysis, including flow cytometric applications, NMR-based techniques, and biochemical and cytometric techniques. Part IV details cultivation techniques, such as disposable bioreactors, hollow fiber cell culture, fixed bed reactors, and configuration of bioreactors. Part V covers downstream techniques such as membrane filtration techniques, while Part VI describes special applications, including retroviral vectors.
Animal Cell Biotechnology: Methods and Protocols, Second Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.
Books:
- Death by Black Hole: And Other Cosmic Quandaries
- Discovering Natural Israel
- Environmentality: Technologies of Government and the Making of Subjects (New Ecologies for the Twenty-First Century)
- Essential Cell Biology, Second Edition
- Ever Since Adam and Eve: The Evolution of Human Sexuality
- Evolution: A Theory In Crisis
- Fish! Tales: Real-Life Stories to Help You Transform Your Workplace and Your Life
- Follow the Drinking Gourd
- Food Fight: The Citizen's Guide to a Food and Farm Bill
- Freshwater Ecology: Concepts & Environmental Applications (Aquatic Ecology)
Books Index
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General Techniques of Cell Culture (Handbooks in Practical Animal Cell Biology)
At the Bench: A Laboratory Navigator
Molecular Cloning: A Laboratory Manual (3-Volume Set)
Lab Ref: A Handbook of Recipes, Reagents, and Other Reference Tools for Use at the Bench
Short Protocols in Molecular Biology (2 volume set)
