Book Description
Flow cytometry continually amazes scientists with its ever-expanding utility. Advances in flow cytometry have opened new directions in theoretical science, clinical diagnosis, and medical practice. The new edition of Flow Cytometry: First Principles provides a thorough update of this now classic text, reflecting innovations in the field while outlining the fundamental elements of instrumentation, sample preparation, and data analysis.
Flow Cytometry: First Principles, Second Edition explains the basic principles of flow cytometry, surveying its primary scientific and clinical applications and highlighting state-of-the-art techniques at the frontiers of research. This edition contains extensive revisions of all chapters, including new discussions on fluorochrome and laser options for multicolor analysis, an additionalsection on apoptosis in the chapter on DNA, and new chapters onintracellular protein staining and cell sorting, including high-speed sorting and alternative sorting methods, as well as traditional technology. This essential resource:
- Assumes no prior knowledge of flow cytometry
- Progresses with an informal, engaging lecture style from simpleto more complex concepts
- Offers a clear introduction to new vocabulary, principles of instrumentation, and strategies for data analysis
- Emphasizes the theory relevant to all flow cytometry, with examples from a variety of clinical and scientific fields
Flow Cytometry: First Principles, Second Edition provides scientists, clinicians, technologists, and students with the knowledge necessary for beginning the practice of flow cytometry and for understanding related literature.
Customer Reviews:
An Expert's Opinion.......2001-09-14
"I consider it to be the best presentation of principles of flow cytometry. The text is clearly written, the figures are well chosen." -- Zbigniew Darzynkiewicz, Brander Cancer Research Institute, New York Medical College
a necessary primer in flow-cytometry.......1997-04-26
A core book designed mainly for the nonspecialist, "Flow Cytometry: First Principles" is addressing in ten chapters the essential issues in flow-cytometry, from flow machines to reagents, techniques and their applications in fundamental research and clinical settings.
The first chapters conveys us as back as the beginning of the century, when the concept of flow-cytometry germinated. Light microscopy, cell counters and ink jet technology are standing at the grounds of flow-cytometry and their merging paved the way toward the modern, "black box", flow cytometers.
However, "beyond the black box", the instrumentation and the technical aspects are comprehensively described in several chapters.
a discussion on data acquisition and analysis is provided, along with the most used cell staining procedures, fluorochromes, lenses, mirrors and lasers. A widespread application in flow-cytometry is cell labelling, particularly leukocyte subpopulations identification with monoclonal antibodies binding cell surface specific markers.
Although lymphocytes are the prototype, methods presented here -dot-plot, histogram, quadrant analysis and gating are applicable to many
other cell types , including bacteria or algae.
With the advent of some "state of the art" machines, DNA and chromosome analyses become feasible by flow-cytometry. It is foreseeable that molecular biology on flow-cytometer will not stop
to cell cycle, ploidy and apoptosis investigation, but even chromosomal translocations or point mutations would be detectable with enough precision for diagnosis.
Beside fundamental research applications, flow-cytometry is now an indispensable technique in the clinical laboratory, too. Oncology,
immunology, hematology and pathology are fields where flow diagnosis has sometimes the last word to say. Cross-match analysis and HLA
phenotyping can now rapidly be performed by flow-cytometry, with usefulness in organ and bone-marrow transplantation.
The final chapter is intended to present the flow-cytometry of the future, as seen in 1992, with an emphasis on the functional assays which are gaining now an increasing interest. However, a
precedent, "out of the stream" chapter is preparing us on what this future could look alike. It approaches some of the border-line pplications, in fields where flow-cytometry is becoming a current tool of research. These are microbiology -prokaryotic DNA and drug resistance determination-, ecology -aquatic environment, plankton, algae-, developmental biology or the functional studies -Ca2+influx.
The book is highly recommended for flow-cytometry and sorting beginners, but it is an equally pleasant lecture for people working
in many life sciences fields, eager to find out how flow-cytometry is shaping current biological research.
Book Description
Synopsis
The Rough Guide to Europe 2005 edition is the ultimate budget guide to the continent. There is a 24-page full-colour section containing a selective and subjective taste of the continent's highlights illustrated with dozens of photos. There are lively accounts of all the main attractions, from seeing the midnight sun to taking a Turkish bath. First-hand advice guides the reader around bar-hopping in Berlin, exploring temples in Greece and trekking in Morocco. The practical focus is on budget travel, with everything students and backpackers need to know to get the most from a trip covering everything from InterRail passes and budget airlines to cycling.
Customer Reviews:
Good and Bad points.......2005-07-24
I took this guide to Europe recently. A lot of the recommendations were good, but the guide is not totally useful while on the trip. For instance, the maps that they provide always seemed to cutoff where I was staying, or don't include all of the train stations, so you don't know how to orient yourself without getting a better map. I realize that the maps focus on the more touristed parts of town, but they do not mark the recommendations on the map, so you're left with an address, and a crappy map that doesn't list streets very well. Rick Steves has the tourist area maps, but he marks his recommendations on his maps, making it much easier to actually use the guide. The Rough Guide makes a lot of good recommendations, it's not as quaint as Rick Steves, which was good for me, but Rick Steves guides are much more user friendly. Whenever we tried to find something using his guide we found it. With the Rough Guide we only found it by chance, or through looking into it on our own at a tourist offfice.
Indispensable Guide For Independent Budget Travellers.......2005-02-03
I used the Rough Guide Europe as my guidebook for a three-month trip through twelve countries. There are seven advantages the Rough Guide has over its competitors:
1) The breadth of coverage -- 32 countries with info on tons of cities and towns, reviews/descriptions of the sights, and options for hiking, cycling, paragliding, and other adventurous pursuits.
2) Thorough listings and reviews for accommodation (hostels primarily, but also hotels and B&Bs), eating, drinking, and nightlife.
3) It's small and weighs next-to nothing.
4) The intuitive, aesthetically-pleasing, user-friendly layout makes it easy to find what you're looking for.
5) Fantastic maps.
6) Everyone else seems to use either Let's Go or Lonely Planet -- this is a tremendous advantage.
7) Loaded with travel details that include basic timetables for trains, buses, and ferries.
This guide book is designed for those who seek to get off the beaten path, and explore Europe on their own. Rough Guides have never let me down. More to the point, they have enriched my travels more than any other guidebook available.
If you are an independent budget traveler planning on a trek to Europe, you would be hard-pressed to find a better guidebook.
Excellent when used properly!.......2004-07-20
The Rough Guide to Europe is excellent in providing a lively overview of every country in Europe (plus Morocco). Rough Guide is unafraid to put in the good with the bad, giving a clear critical analysis of what's worth seeing and what isn't. All the cities and destinations one would expect to find--from Slovenian resorts to Paris to Santiago de Compostela--are included in here.
Of course, putting all the relevant information for the whole of Europe would require several dozen 2000-page travel guides, so travelers to a specific country will also want to pick up one of Rough Guide's country-specific books. Yet if I had to backpack across the whole of Europe with a single book, this would be it!
The maps for such cities as Sevilla, Venice, and Lisbon are excellent for the brief visitor, and really give a feel of how to distribute your all-too-often limited time.
If there is any minor issue I have with this guide, it is that the hotel selections are limited and generally inferior to what you might find in a full guide to a particular city. Of course, this goes back to having to squeeze in several dozen nations in one book.
All in all, if you are planning on visiting several countries or just meandering through Europe, Rough Guide to Europe is a one great buy.
Rough Guide is a must for European Trip.......2004-03-28
What I like best about the Rough Guides is that they give critical reviews. They will point out the tourist traps and will give negative reviews. I find that Frommer's and Fodor's rarely point out the negatives to a particular... The rough guide is very critical and is a great balance to these other guides.
If you aren't interested in "roughing" it and staying in lower priced hotels.. the guides are still very useful in rating attractions, and areas in which to stay... but you will need another book to look at more moderate and luxury hotels.
I would definitely read this book before going to Europe.
Amazon.com
Eighty-five years after a famous but ill-equipped Canadian Arctic expedition of 1913 had sacrificed 16 lives, some artifacts appeared on an Internet auction site. They had originated at a "ghost camp," discovered in 1924, where four of the expedition's 28 men, one woman, and two children had perished. Jennifer Niven has completed the unfulfilled mission of survivor William McKinlay to produce a "more honest and revealing account" of the wreck of the Karluk and its aftermath.
The explorers became split into several dispersed groups living "in the shadow of death." Their simultaneously grim and gruesome experiences are interwoven in this minutely detailed and atmospheric retelling, created by combining and comparing firsthand accounts and other sources. The characters are vividly re-created, from the expedition's self-interested leader, whom McKinlay called "a consummate liar and cheat," to the heroic ship's master, who struggled over 700 miles to organize a rescue. Supplemented by haunting and fascinating photographs, The Ice Master makes for harrowing and compulsive reading. This is a momentous story of the Arctic; of adventure, misadventure, and the heights of human endurance. But it is also a story of human failings and the waste of young lives, as poignant now as it was when it was big news in 1914. --Karen Tiley, Amazon.co.uk
Book Description
Eighty-five years after a famous but ill-equipped Canadian Arctic expedition of 1913 had sacrificed 16 lives, some artifacts appeared on an Internet auction site. They had originated at a "ghost camp," discovered in 1924, where four of the expedition's 28 men, one woman, and two children had perished. Jennifer Niven has completed the unfulfilled mission of survivor William McKinlay to produce a "more honest and revealing account" of the wreck of the Karluk and its aftermath.The explorers became split into several dispersed groups living "in the shadow of death." Their simultaneously grim and gruesome experiences are interwoven in this minutely detailed and atmospheric retelling, created by combining and comparing firsthand accounts and other sources. The characters are vividly re-created, from the expedition's self-interested leader, whom McKinlay called "a consummate liar and cheat," to the heroic ship's master, who struggled over 700 miles to organize a rescue. Supplemented by haunting and fascinating photographs, The Ice Master makes for harrowing and compulsive reading. This is a momentous story of the Arctic; of adventure, misadventure, and the heights of human endurance. But it is also a story of human failings and the waste of young lives, as poignant now as it was when it was big news in 1914. --Karen Tiley, Amazon.co.uk
Download Description
The riveting story of the 1913 expedition of twenty-five people who sailed out of British Columbia in search of an undiscovered Arctic continent. But tragedy struck in January 1914 when an ice cap tore a hole in the vessel's hull, shipwrecking all on board. The castaways abandoned ship and suffered from battle starvation, snow blindness, exposure to the brutal winter, and each other. The Ice Master is an epic tale of true adventure that rivals the most dramatic fiction. Drawing on the diaries of those who were rescued and those who perished, and even an interview with the one living survivor, Jennifer Niven re-creates with astonishing accuracy and immediacy the Karluk's ill-fated journey and her crew's desperate attempts to find a way home from the icy wastes of the Arctic.
Customer Reviews:
Great Book!.......2007-08-17
The start of this book was a little rough for me. The author introduced all the character all at once and I was pretty confused for a while, but once you get into the story you're hooked! This book was like a great episode of Dateline or 20/20. It was full of excitement, mystery, and really kept you on the edge of your seat wondering what happened and what was going to happen. It would be great to take todays forensics to figure out some of the things they couldn't figure out back then...sadly most of the artifacts from the doomed Karluk expedition have been lost.
Poorly written.......2007-03-07
Coincidentally I had just finished 'Alive' - another story about cold weather survival. Perhaps mainly by contrast I found it impossible to get into 'The Ice Master'. While the event is undeniably interesting, Niven's telling ruins it.
Another amazing arctic adventure story.......2006-04-04
I love reading the stories of the great arctic adventures...This one is awesome! It's basiclly the opposite of Shackeltons Endurance...No comradery, lots of death, a coward for a leader and even at the other end of the world. It really offers up a contrast. Yet through all the struggles heros still arise to meet the challenge.
ABSOLUTELY ENTHRALLING!.......2005-08-03
I absolutely could not put this book down. We had a house full of family guests and I found myself sneaking away just to try and grab a few more pages of this thrilling, yet horrifying account of the voyage of the Karluk. On one hand, I almost dreaded to pick the book up because Niven truly brings these characters to life and you become very involved with them. On the other, I just had to know who would and who would not survive and how the ones who did survive managed to do it. Niven did a superb job all around.
Another great Artic adventure, on par with "South".......2005-03-24
For me "South", is the classic Polar expedition tragedy book. This book is on par with it. However in any second source history book, there are sometimes some niggling details that are wrong. The fact that another "doomed" expedition had some survivers, may not have been known to the crew on this one. But its not clear that is what happened. Now I want to go find that book and read it. But had you only read the original books & sources you would have come away with a completely different perspective. Ms. Niven got to the heart of the trip and who were really the heros here and who were the cads.
But, this is more than just a man triumphs over nature book. Some of these folks survived but it wasn't great. It was a good read though and I'm glad I picked it up. I'm going after Ms. Niven's other book "Ada" next.
Average customer rating:
- trouble in sabah
- Adventure Training Disaster
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Descent into Chaos: The Doomed Expedition to Low's Gully
Richard Connaughton
Manufacturer: Potomac Books
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ASIN: 1857531477 |
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trouble in sabah.......2002-02-12
Saw half the movie, didn't read the book yet. What is the title of the movie ? Is it for sale ?
Adventure Training Disaster.......2000-07-30
This true story of survival has been the subject of two other books and a movie. In 1994, a ten-man group of soldiers, 7 British and 3 Chinese from Hong Kong, went to Sabah, Malaysia with the intention of being the first ever to successfully navigate Lows Gully. Lows Gully is a deep chasm off the northern flank of Mount Kinabalu. Superstition, mystery and intrigue surrounds this place instilling fear into some of the locals. It was into this situation that these men went to create history.
The other two books that were written about this expedition came from four of the team involved. With regard to this book, R.M. Connaughton is independent. He seems well qualified to undertake such a task having a military background and actually serving in the Far East. At the beginning of the book, he provides historical, yet essential background information to Mount Kinabalu and Lows Gully. He relates the exploits of Hugh Low (whom Lows Gully was named after) and Spenser St John. In the 1990's the attempt by Robert New and Steve Pinfield is also related.
With the background information, Connaughton then begins to describe the preparations for the expedition describing the building of the team and the travelling to Mount Kinabalu. Their is a photo section in the middle of the book and at the end there is a chapter entitled "Reflections" which describes some of the findings of the subsequent Board of Enquiry.
The book highlights the various tensions that existed between team members and tries to establish what exactly went wrong. Connaughton does an excellent job with this difficult task. The chapter detailling the extensive rescue operation that took place with the British and Malaysians deserves a special mention.
This is an excellent book and you can almost envisage the savage jungle terrain that is described as you read it.
On the whole this is a well-written and well researched book and I thoroughly recommend it.
Book Description
Ponting happily agreed to document Scott's upcoming South Pole expedition, which turned out to be Scott's final trek.
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Arctic Experiences: Aboard the Doomed Polaris Expedition and Six Months Adrift on an Ice-Floe
George E Tyson
Manufacturer: Cooper Square Press
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ASIN: 0815411898 |
Book Description
In 1871 veteran navigator George Emory Tyson joined the first American expedition to explore the North Pole, but by August 1872, after the mysterious death of the Polaris' leader, Charles Francis Hall, the ice pack in which the ship was travelling began to break up. Nineteen crew members, including Tyson, were stranded on an ice-floe when the ship abandoned them. They were not rescued until April 30, 1873. Unavailable for nearly a century, Arctic Experiences is the fierce, harrowing chronicle of Tyson's incredible feat of endurance and survival.
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Divided Loyalties in a Doomed Empire: The French in the West from New France to the Lewis and Clark Expedition
Daniel Royot
Manufacturer: University of Delaware Press
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The Doomed Expedition
Jack Adams
Manufacturer: Arrow (A Division of Random House Group)
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Doomed Expedition: The Norwegian Campaign of 1940
Jack Adams
Manufacturer: Leo Cooper
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ASIN: 0850520363 |
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Doomed Expeditions (Incredible Histories)
John Duggleby
Manufacturer: Crestwood House
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ASIN: 0896865061 |
Books:
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