Using Antibodies : A Laboratory Manual : Portable Protocol NO. I

Book Description

Few technical manuals have become standards in biomedicine. Antibodies: A Laboratory Manual, by Ed Harlow and David Lane, has had that status for a decade. Now there is a new and even higher standard—Using Antibodies: A Laboratory Manual.

Harlow and Lane have completely revised their guide to the use of immunoglobulin reagents in the laboratory. Chapters have been entirely rewritten, reorganized, and updated to provide background, context, and step-by-step instructions for techniques that range from choosing the right antibody and handling it correctly, to the proper methods for characterizing antigens in cells and solutions. New chapters on tagging proteins and epitope mapping are included.

Rather than presenting an array of solutions for working with antibodies and antigens, Using Antibodies instead identifies in each case the best approach to specific problems. These recommendations include more detail in the protocols, extensive advice on avoiding and solving problems, information regarding proper controls, and extensive illustration of theory, methods, and results, both good and bad. An additional bonus included with this manual is a set of Portable Protocols, step-by-step instructions for the most frequently used and essential techniques printed on spill-proof, durable cards that can be annotated and used directly at the bench.

The expert advice in Using Antibodies is presented using an imaginative design with extensive use of color and graphic elements calculated to help readers plan and execute their experiments efficiently and accurately. A newly available type of binding will maintain the manual's integrity during years of use.

Customer Reviews:

Should be [less money], Not [more money]!.......2002-10-21

Although I liked the outdated 1988 version of this book, this one is extremely simplistic and omits much of the previously included material. Most of the information included can be found on the web and is shallow compared to Current Protocols or other texts. Although it serves as a reasonable introduction for novices to this area it's extremely overpriced for [money]!!!

I would reccomend Monoclonal Antibodies by Goding ([money]),

Two Thumbs Up.......2000-10-25

This is an exceptional reference for anyone using abtibodies in the laboratory. It is a *must-have* for anyone venturing into unknown teritory. For the experienced individual forced to bang out new protocols with a cranky antibody, this reference is priceless.

Immunochemical Protocols (Methods in Molecular Biology (Cloth))

Average customer rating:

Immunochemical Protocols (Methods in Molecular Biology (Cloth))
Robert, Ed. Burns
Manufacturer: Humana Press
ProductGroup: Book
Binding: Hardcover

Biochemistry | Biological Sciences | Science | Subjects | Books

General | Science | Subjects | Books

Biochemistry | Basic Science | Medicine | Subjects | Books

Immunology | Basic Science | Medicine | Subjects | Books

All Titles | Qualifying Textbooks - Fall 2007 | Stores | Books
ASIN: 1588292746

Book Description

This new edition of a much praised classic includes both basic method for producing and using antibodies and advanced techniques required by today's state-of-the art investigators. The basic sections of the book stress the first principles of antibody production and guide the reader through the processes and pitfalls of immunochemistry. The advanced sections focus on specific applications and cover the immunochemical techniques now used in laboratories. New material includes techniques for making new monoclonal cell lines, for producing good quality monoclonal antibodies, immunocapture PCR, immunoaffinity purification, and immunoelectron microscopy in plant virus diagnosis.

Average customer rating:

Good manual, though needs revision.
An old classic, but still useful.
An excellent summary of immunological methods.

Antibodies
Harlow
Manufacturer: Cold Spring Harbor Laboratory Press
ProductGroup: Book
Binding: Paperback

Research | Education | Science | Subjects | Books

Experiments & Projects | Experiments, Instruments & Measurement | Science | Subjects | Books

Methodology & Statistics | Experiments, Instruments & Measurement | Science | Subjects | Books

General | Science | Subjects | Books

Immunology | Basic Science | Medicine | Subjects | Books

Microbiology | Basic Science | Medicine | Subjects | Books

General | Research | Medicine | Subjects | Books

Research | Medical | Professional & Technical | Subjects | Books

jp-unknown2 | Specialty Stores | Books

All Titles | Qualifying Textbooks - Fall 2007 | Stores | Books

Medicine | Qualifying Textbooks - Fall 2007 | Stores | Books

Professional | Qualifying Textbooks - Fall 2007 | Stores | Books

Science | Qualifying Textbooks - Fall 2007 | Stores | Books
Similar Items:

Molecular Cloning: A Laboratory Manual (3-Volume Set)
Using Antibodies : A Laboratory Manual : Portable Protocol NO. I
Manipulating the Mouse Embryo: A Laboratory Manual
Culture of Animal Cells: A Manual of Basic Technique
Short Protocols in Molecular Biology (2 volume set)

ASIN: 0879693142

Customer Reviews:

Good manual, though needs revision........2007-06-28

This laboratory manual is very concise and easy to read. The topics range from antibody production to protein/enzyme conjugation, with step by step instructions and formulations. Previous to most topics is given a fairly thorough background. One drawback is the fact that this book is nearly 20 years old (pub. 1988) and lacks a significant amount of progress made in the areas of homo and heterobifunctional linkers, PEGylation, and antibody engineering. Given a good price however, this would be a good reference manual.

An old classic, but still useful........2002-08-26

This classic is getting old...
But you cand find some important informations inside, classic protocols and keys-data to the antibodies-world.
A must-have reference for any today-biologist working with antibodies (immunostaining, immunoprecipitation, Western-blot, antibodies production, etc...).

An excellent summary of immunological methods........1998-01-16

This book is indespensible for those wishing to set up any type of antibody related methodology. Gives excellent overviews of immunodiagnostic methods (ELISA,RIA,etc.), antibody production (polyclonal and monoclonal), and includes basic summaries on the nature of the immune sytem in vivo. Especially useful to researchers developing immuno-techniques as it gives detailed methodologies.

Antibody Engineering: A Practical Approach (Practical Approach Series)

Average customer rating:

Antibody Engineering: A Practical Approach (Practical Approach Series)

Manufacturer: Oxford University Press, USA
ProductGroup: Book
Binding: Paperback

Genetics | Evolution | Science | Subjects | Books

General | Pharmacology | Medicine | Subjects | Books

Biotechnology | Special Topics | Medicine | Subjects | Books

General | Health, Mind & Body | Subjects | Books

All Titles | Qualifying Textbooks - Fall 2007 | Stores | Books
ASIN: 0199635927

Book Description

Recombinant DNA techniques have revolutionized the isolation and production of antibodies in recent years, and rapid changes have been made. This new Practical Approach book details protocols which allow the researcher to isolate a new antibody, analyse its properties, format the right antibody molecule or fragment, and produce it in usable quantities.

Techniques in Prion Research (Methods and Tools in Biosciences and Medicine)

Average customer rating:

Techniques in Prion Research (Methods and Tools in Biosciences and Medicine)

Manufacturer: Birkhäuser Basel
ProductGroup: Book
Binding: Paperback

Research | Education | Science | Subjects | Books

Methodology & Statistics | Experiments, Instruments & Measurement | Science | Subjects | Books

General | Science | Subjects | Books

Immunology | Basic Science | Medicine | Subjects | Books

General | Diseases | Medicine | Subjects | Books

General | Medicine | Subjects | Books

General | Research | Medicine | Subjects | Books

Research | Medical | Professional & Technical | Subjects | Books

All Amazon Upgrade | Amazon Upgrade | Stores | Books

Medicine | Amazon Upgrade | Stores | Books

Professional & Technical | Amazon Upgrade | Stores | Books

Science | Amazon Upgrade | Stores | Books

All Titles | Qualifying Textbooks - Fall 2007 | Stores | Books

Medicine | Qualifying Textbooks - Fall 2007 | Stores | Books

Professional | Qualifying Textbooks - Fall 2007 | Stores | Books

Science | Qualifying Textbooks - Fall 2007 | Stores | Books
ASIN: 3764322241

Book Description

Prion diseases, also known as Transmissible Spongiform Encephalopathies (TSEs), exist in both humans (Creutzfeldt-Jakob disease (CJD)) and animals (scrapie, bovine spongiform encephalopathy (BSE), chronic wasting disease) and have the unique property of being infectious, sporadic or genetic in origin.

Although the precise nature of the infectious agent responsible for TSEs is not definitely identified, it is now clearly demonstrated that a protein named PrP (for Prion Protein) plays a critical role in the transmission and pathogenesis of TSEs.

This book provides the general description as well as the details of the techniques currently used for the study of prion diseases. Taking into account the pivotal role played by PrP it is not surprising that many Chapters of this book deal with the purification, the detection and the characterization of the different forms of this protein. In addition, in vitro, cellular and animal models specifically adapted to the study of TSEs, as well as bio-safety procedures are described. Each Chapter is written by scientists involved for many years in their respective domain of prion biology who give the best of their knowledge in this technical document. This volume is a very useful tool for any laboratory which recently decided to contribute to the study of TSEs as well as for teams already engaged in this field for many years but interested in extending their technical capacity toward new methods.

Features:

Purification of PrPC and the Pathological Isoform of Prion Protein (PrPsc or PrP-res)

Animal Models of TSEs

Cell Culture Models of TSEs

PrPsc Immunohistochemistry

Western Immunoblotting Techniques

Antibody Production and ELISA

TSE Strain Typing in Mice

Biosafety and Decontamination Procedures

Cell-free Conversion of Prion Proteins

Cytotoxicity of PrP Peptides

Cyclic Amplification of Prion Protein Misfolding

Of interest to:

Researchers and clinicians in the fields of cell biology, biomedicine, neuroscience/neuropathology, veterinary medicine and biochemistry.

Manual of Diagnostic Antibodies for Immuniohistology

Average customer rating:

Manual of Diagnostic Antibodies for Immunohistology

Manual of Diagnostic Antibodies for Immuniohistology
Anthony S-Y Leong
Manufacturer: Greenwich Medical Media
ProductGroup: Book
Binding: Hardcover

Immunology | Basic Science | Medicine | Subjects | Books

General | Medicine | Subjects | Books

All Titles | Qualifying Textbooks - Fall 2007 | Stores | Books

Medicine | Qualifying Textbooks - Fall 2007 | Stores | Books

Professional | Qualifying Textbooks - Fall 2007 | Stores | Books
Similar Items:

Diagnostic Immunohistochemistry

ASIN: 1841101001

Book Description

The rapid acceptance of immunohistochemistry as an important and even indispensable adjunct to morphological examination and diagnosis requires the modern anatomical pathology laboratory to be conversant with, and proficient in, immunostaining procedures, as well as methods of tissue processing and antigen retrieval and the underlying characteristics of the increasing number of antibodies and antisera available. This fully revised, expanded and updated edition provides a comprehensive list of antisera and monoclonal antibodies that have useful diagnostic applications in tissue sections and cell preparations. Various clones, which are commercially available to detect the same antigen, are listed and the sensitivities and specificities of the antibodies are discussed. Importantly, the authors, all pathologists with a wealth of experience in immunostaining procedures, provide fully referenced details and expert advice on each reagent, which the reader will find invaluable. Finally, the appendices provide easily accessible and clear summaries of selected antibody panels for specific diagnostic situations, details of heat-induced antigen/epitope retrieval (including the use of microwaves) and a useful reference to the websites of the main antibody suppliers.

Customer Reviews:

Manual of Diagnostic Antibodies for Immunohistology.......2006-11-03

This manual is excellent for an immunohistochemistry laboratory. The information on antibody clones and proper control tissue is fantastic.

Chicken Egg Yolk Antibodies, Production And Application: IGY-Technology (Springer Lab Manual)

Average customer rating:

Chicken Egg Yolk Antibodies, Production And Application: IGY-Technology (Springer Lab Manual)
Rudiger, Ed. Schade
Manufacturer: Springer
ProductGroup: Book
Binding: Spiral-bound

Biotechnology | Biological Sciences | Science | Subjects | Books

General | Science | Subjects | Books

Immunology | Basic Science | Medicine | Subjects | Books

Biotechnology | Special Topics | Medicine | Subjects | Books

General | Medicine | Subjects | Books

General | Research | Medicine | Subjects | Books

Research | Medical | Professional & Technical | Subjects | Books

All Amazon Upgrade | Amazon Upgrade | Stores | Books

Engineering | Amazon Upgrade | Stores | Books

Medicine | Amazon Upgrade | Stores | Books

Professional & Technical | Amazon Upgrade | Stores | Books

Science | Amazon Upgrade | Stores | Books
Accessories:

Immune Regulation and Immunotherapy in Autoimmune Disease
Mechanisms of Lymphocyre Activation and Immune Regulation XI: B Cell Biology (Advances in Experimental Medicine & Biology)
The Papillomaviruses

ASIN: 3540666796

Book Description

Polyclonal antibodies, widely used in research and diagnostics, are conventionally isolated from the blood of immunised mammals. The fact that antibodies can also be detected in the yolk of eggs laid by immunised hens, led to the development of the yolk antibody technology as an alternative method less stressful to animals. Hens can be kept under nearly natural conditions and antibodies be isolated from the eggs. Further, the IgY-technology has the advantage that avian antibodies can be produced against several antigens which do not induce a immune response in mammals. Protocols on how to keep and immunise hens and on the extraction, isolation and use of antibodies from yolk are described in detail in this manual. For their work on yolk antibodies the authors received the FISEA (International Foundation for the Substitution of Animal Experimentation, Luxembourg) prize in 1997.

Monoclonal Antibodies: A Practical Approach (Practical Approach Series)

Average customer rating:

Monoclonal Antibodies: A Practical Approach (Practical Approach Series)
Philip S., Ed. Sheperd
Manufacturer: Oxford University Press
ProductGroup: Book
Binding: Paperback

Immunology | Basic Science | Medicine | Subjects | Books

General | Medicine | Subjects | Books

All Titles | Qualifying Textbooks - Fall 2007 | Stores | Books
ASIN: 0199637229

Book Description

Monoclonal Antibodies: A Practical Approach covers the preparation, testing, derivation, and applications of monoclonal antibodies. New immunological techniques incorporating tried and tested methodologies are described, making the book of interest to established and inexperienced immunologists. Both the standard somatic hybridization technique and recombinant techniques, including the use of phage libraries, for the preparation of rodent and human monoclonal antibodies are described. Protocols for both the small and large scale production are detailed, as well as purification and labelling (with both radioisotopes and non-radioisotopes) methods. The applications of monoclonal antibodies in immunoblotting, enzyme linked immunoassays, immunofluorescence, and FACS analysis are all covered in detail. Finally protocols are given for the use of monoclonal antibodies in rheumatoid arthritis, tissue typing, detecting DNA modified during chemotherapy, and in the clinical analysis of transplantation samples for malignancy. This book will therefore be an invaluable laboratory companion to anyone using monoclonal antibodies in their research.

Monoclonal Antibodies: Methods and Protocols (Methods in Molecular Biology (Cloth))

Average customer rating:

Monoclonal Antibodies: Methods and Protocols (Methods in Molecular Biology (Cloth))
Maher, Ed. Albitar
Manufacturer: Humana Press
ProductGroup: Book
Binding: Hardcover

General | Science | Subjects | Books

Immunology | Basic Science | Medicine | Subjects | Books

General | Medicine | Subjects | Books

All Titles | Qualifying Textbooks - Fall 2007 | Stores | Books
ASIN: 1588295672

Book Description

Monoclonal Antibodies: Methods and Protocols examines a collection of state-of-the-art methods that employ monoclonal antibodies in a clinical setting with opening chapters focusing on the gold standard method for generating mouse monoclonal antibodies through hybridoma technology, future methods for engineering recombinant and humanized antibodies, methods for engineering soluble Fc fusion protein, and the use of antibodies and flow cytometry in the quantification of cell signaling proteins.

Specific chapters describe how antibodies are used for the diagnosis and classification of hematologic diseases. Subsequent chapters examine the advantages and most recent advances of using bead-based immunoassays, including the ability of bead-based technology to multiplex and analyze several analytes simultaneously, and the use of beads in detecting fusion proteins resulting from chromosomal translocations. Concluding chapters provide additional examples of methodologies that employ monoclonal antibodies.

Monoclonal Antibodies: Methods and Protocols provides descriptions of methods that cover a wide spectrum of applications in the field of monoclonal antibodies. This field will continue to expand and provide new and innovative techniques, not only in the laboratory, but also as a basis that complements targeted therapy.

Molecular Probes of the Nervous System: Selected Methods for Antibody and Nucleic Acid Probes (Molecular Probes of the Nervous System) (Molecular Probes of the Nervous System)

Average customer rating:

Molecular Probes of the Nervous System: Selected Methods for Antibody and Nucleic Acid Probes (Molecular Probes of the Nervous System) (Molecular Probes of the Nervous System)
Susan Hockfield , Steve Carlson , and Chris Evans
Manufacturer: Cold Spring Harbor Laboratory Press
ProductGroup: Book
Binding: Plastic Comb

Research | Education | Science | Subjects | Books

Experiments & Projects | Experiments, Instruments & Measurement | Science | Subjects | Books

Methodology & Statistics | Experiments, Instruments & Measurement | Science | Subjects | Books

General | Science | Subjects | Books

Physiology | Basic Science | Medicine | Subjects | Books

General | Medicine | Subjects | Books

Diagnosis | Physician & Patient | Medicine | Subjects | Books

Books:

Books Index

Books Home

Recommended Books