Fly Pushing: The Theory and Practice of Drosophila Genetics

Book Description

A new edition of the classic handbook that has become a standard in the Drosophila field. The book is expanded to include topics in which classical genetic strategies have been augmented with new molecular tools. Included are such new techniques as homologous recombination, RNAi, new mapping techniques, and new mosaic marking techniques.

Customer Reviews:

Great for Drosophila work or class over this model system.......2007-02-08

I'm a first year grad student, and I can't say enough good things about this book! It was extremely helpful in my lab rotation as well as my upper level genetics course (covered genetic model systems)

The Bible of the Fly Pusher!!!!!.......2002-08-26

A really must-have!!!
The basics, the tips,
all for manipulating drosophila at the beginning and even later.
Here you'll find a lot of informations about balancers, recombinations, how to do crosses, markers, etc...
A very good introduction to the drosophila world!

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Comprehensive in scope, not quite enough detail.
Comprehensive in scope, not quite enough detail.
A must have for any Drosophila lab

Drosophila Protocols
William Sullivan
Manufacturer: COLD SPRING HARBOR LABORATORY PRESS
ProductGroup: Book
Binding: Hardcover

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ASIN: 0879695862

Book Description

This exceptional laboratory manual describes thirty-seven procedures most likely to be used in the next decade for molecular, biochemical, and cellular studies on Drosophila. They were selected after extensive consultation with the research community and rigorously edited for clarity, uniformity, and conciseness.

The outstanding features of this protocol collection are:

- Scope: The methods included permit investigation of chromosomes, cell biology, molecular biology, genomes, biochemistry, and development

- Depth: Each protocol includes the basic information needed by novices, with sufficient detail to be valuable to experienced investigators.

- Format: Each method is carefully introduced and illustrated with figures, tables, illustrations, and examples of the data obtainable.

- Added value: The book's appendices include key aspects of Drosophila biology, essential solutions, buffers, and recipes.

An evolution of Michael Ashburner's 1989 classic Drosophila: A Laboratory Manual, this book is an essential addition to the personal library of Drosophila investigators and an incomparable resource for other research groups with goals likely to require fly-based technical approaches.

Customer Reviews:

Comprehensive in scope, not quite enough detail........2000-06-23

This book is very impressive in scope, and (as other reviewers have said) IS indispensable for any Drosophila laboratory. It is a very valuable addition to every Drosophila laboratory. The book covers virtually every area of Drosophila work. Many of the topics discussed are cutting edge, such as the chapter on RNA interference, targeted cell ablation, and biochemical preparations from Drosophila (such as soluble nuclear extracts and membrane proteins). Overall I am very pleased with the book, and glad I bought it! One section of the book I find particularly useful is the beginners guide to the Drosophila genome project. This section overviews the information available from the recently completed Drosophila genome project. It tell us what kinds of information are available about the fly genome, how this information was collected, and where we can download it from the internet (complete with internet web site addresses)!

My only criticism of the book is a criticism which applies to ALL manuals published by the Cold Spring Harbor Laboratory Press: the protocols are too superficial, and not dealt with in sufficient detail to allow a novice to carry out these techniques (which is what a manual ideally SHOULD do). This has been true of all the CSHLP manuals I have seen. Conspicuously absent from the CSHL publications is a TROUBLESHOOTING section, which can be referred to if the researcher runs into problems. As we all know, there are many small (and seemingly trivial) details to any protocol which, if not carried out properly, can make or break an experiment. Some steps in a protocol are always more fastidious than others, and it is critical for us to know which steps can be done with a bucket and which steps require an eyedropper.

For my money, the best and most comprehensive manual is the CURRENT PROTOCOLS IN MOLECULAR BIOLOGY manual (Wiley and Sons), which is updated regularly, and has extensive troubleshooting sections attached to each protocol. (They have recently come out with a companion manual dealing exclusively with protein techniques.) Also, I highly recommend the METHODS IN MOLECULAR BIOLOGY series (Humana Press) for its concise protocols and supplementary troubleshooting sections. If the researcher is familiar with the techniques the troubleshooting sections need not be consulted, but if the researcher is a novice the troubleshooting sections are invaluable. Unfortunately a Drosophila manual is not available from either of these publishers, but this CSHL manual makes a good substitute. I recommend this book for all Drosophilists!

Comprehensive in scope, not quite enough detail........2000-06-23

The complete index of DROSOPHILA PROTOCOLS (CSHLP) is available at the publishers website given below...

A must have for any Drosophila lab.......2000-04-20

This latest edition by Sullivan et al. is the best protocols manual for Drosophila that I've seen. It should be a staple for any fly lab. It provides the most up-to-date protocols for common techniques (ie staining, dissections, cytology) as well as more recent additions (i.e. DNA microarrays, neuroactive drugs, GFP reporters). Moreover, the protocols are clear, detailed, and easy to follow.

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Drosophila: A Laboratory Handbook
Michael Ashburner
Manufacturer: Cold Spring Harbor Laboratory Press
ProductGroup: Book
Binding: Hardcover

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ASIN: 0879697067

Book Description

The second edition of the highly successful "grey book" rosophila: A Laboratory Handbook, first published in 1989, has been completely updated to reflect advances in techniques and knowledge. The original format is maintained, providing a handy reference guide to most aspects of the biology of Drosophila. Each of the thirty-six chapters summarizes the present state of the subject, written with a historical perspective, but with an emphasis on the practical use of genetic and other methods. Extensively referenced.

Drosophila: A Practical Approach (Practical Approach Series)

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Good book, but biased in favour of genetics:

Drosophila: A Practical Approach (Practical Approach Series)

Manufacturer: Oxford University Press, USA
ProductGroup: Book
Binding: Paperback

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ASIN: 0199636605

Book Description

Drosophila--the scientist's favorite laboratory organism--is a key subject for studying genetics, development, and behavior. The second edition of this popular hands-on guide brings the methodology up-to-date, with contributions from the leading researchers in the field. Chapters include the
elements of Drosophila biology and genetics; mutagenesis; transposons--gene tagging and mutagenesis; chromosome mechanics: the genetic manipulation of aneuploid stocks; enhancer traps; looking at embryos; immunolabelling of Drosophila; population and ecological genetics; behavior, learning, and
memory; cell culture; and preparation of nucleic acids.

Customer Reviews:

Good book, but biased in favour of genetics:.......2000-01-18

This is a very good book, and contains useful protocols. For those of us who remember the first edition, it was heavily biased in favour of genetics. So is the second edition, in spite of the fact that Drosophila is being used more and more for studies in cytology, physiology and biochemistry. Nevertheless, this book is indispensible for those interested in doing classical genetics with Drosophila. It explains how to maintain flies, how to do basic crosses and ballance chromosomes, and how to do P-element induced mutations. There is also a section of how to extract and manipulate nucleic acids from flies, and a very brief (but interesting section) on fly BEHAVIOR and genetic studies of fly behavior. I found this interesting since many researchers have been turning to Drosophila as a model organism in learning and behavior (including addiction and substance abuse) studies in recent years. Several things are noticably lacking from this book however. A section on polytene chromosomes is conspicuously absent, in spite of the fact that this is one of the main tools of Drosophilists. The inclusion of several sections on fly cytology would definitely have been useful, such as PISH (polytene in situ hybridization), and other methods for locating gene products in fly cells. A section on microinjection and transformation of fly embryos would have been useful too. The basic theoretical information is there, but the technique is not dealt with specifically, and no protocols are given. The P-element enhancer trap system is dealt with briefly, but could have been expanded more. For those interested in the fruitfly as a model organism in cytology and molecular biology I would recommend 'Drosophila melanogaster: Practical Uses in Cell and Molecular Biology,' from the 'Methods in Cell Biology' series (Volume 44). This is a good book although it too could use more detailed protocols. Unfortunately this book is out of print, so it is hard to comeby! Alternatively, the 'Cold Spring Harbour Press' is due to come out with 'Drosophila Protocols' in the Spring of 2000. I have seen the putative table of contents, and it looks like this may be a very comprehensive book, and I'll make sure to have a look at it as soon as it is published. Greg Doheny (Vancouver B.C.)

Muscle Development in Drosophila (Molecular Biology Intelligence Unit)

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Muscle Development in Drosophila (Molecular Biology Intelligence Unit)
Helen Sink
Manufacturer: Springer
ProductGroup: Book
Binding: Hardcover

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ASIN: 0387300538

Book Description

The different aspects of muscle development are considered from cellular, molecular and genetic viewpoints, and the text is supported by black/white and color illustrations. The book will appeal to those studying muscle development and muscle biology in any organism.

Drosophila Eye Development (Results and Problems in Cell Differentiation)

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Drosophila Eye Development (Results and Problems in Cell Differentiation)

Manufacturer: Springer
ProductGroup: Book
Binding: Hardcover

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ASIN: 354042590X

Book Description

The compound eye of Drosophila is used as a model for human disease and homology to eyes in other taxa. This book covers the major discoveries on the development of the compound eye of Drosophila melanogaster over the last 25 years. These include aspects of the biological mechanisms of pattern formation in the nervous system, the specification of neuronal cell types, unexpected phylogenetic conservation and many new insights into the function of several signal transduction pathways. All chapters in this book have been written by leading experts in this field who have made significant contributions to our understanding of fly eye development.

Making Of A Fly: GENETICS OF ANIMAL DESIGN

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The Connection Between DNA and The Animal
What knock-outs can tell you
bristling with insight
Self-builder
Good Book for research

Making Of A Fly: GENETICS OF ANIMAL DESIGN
Peter A. Lawrence
Manufacturer: Blackwell Publishing
ProductGroup: Book
Binding: Paperback

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ASIN: 0632030488

Book Description

Understanding how a multicelluar animal develops from a single cell (the fertilized egg) poses one of the greatest challenges in biology today. Development from egg to adult involves the sequential expression of virtually the whole of an organisms genetic instructions both in the mother as she lays down developmental cues in the egg, and in the embryo itself. Most of our present information on the role of genes in development comes from the invertebrate fruit fly, Drosophila. The two authors of this text (amongst the foremost authorities in the world) follow the developmental process from fertilization through the primitive structural development of the body plan of the fly after cleavage into the differentiation of the variety of tissues, organ and body parts that together define the fly. The developmental processes are fully explained throughout the text in the modern language of molecular biology and genetics. This text represents the vital synthesis of the subject that many have been waiting for and it will enable many specific courses in developmental biology and molecular genetics to be focussed upon it appealing to 2nd and 3rd year students in these disciplines as well as in biochemistry, neurobiology and zoology. It will also have widespread appeal amongst researchers.

Customer Reviews:

The Connection Between DNA and The Animal.......2006-06-20

For the uninitiated such as I, it has been entirely mysterious how a strand of DNA manages to express itself as a finished animal. Indeed, I really do believe evolution works, and that the information that describes an individual animal is passed on through the coding of DNA. But it has been uncomfortable accepting this principle on faith because authorities in the field say so. Here is a book that describes the process and explains how scientists detect and measure the mechanism. To all my fellow skeptics, I highly recommend this book.

What knock-outs can tell you.......2005-11-04

This is a wonderful summary of that which we have learned from genetic knock-out and knock-in technology. There is a systemic evaluation of even the earliest cell divisions and the results are clear: knock-out or knock-in a gene, and the patterns change. Even axial organization of the egg can be influenced by knocking out a gene.

The focus is entirely on making sense of the drosophila with knock-out and knock-in experiments . Fundamental research into initiating metabolic activity, surface chemistry and protein assembly is not addressed.

bristling with insight.......2002-02-20

What a great book! Lawrence has managed to take the long history and complex details of genetic studies on drosophila development, and synthesize it all into an accessible summary that anyone can understand. This is by far the most concise and straightforward summary of fly development, and should be considered a must-read for anyone who cares about developmental biology. OK, full-time fly people will probably find it mostly too basic, but for the rest of us it's just right. Sidebars on the different techniques provide useful details for those who care without interrupting the flow of the prose. Defiantely recommended. Plus, the cover picture is really cool.

Self-builder.......2000-11-27

The general reader interested in not only how a single fly egg cell develops into a complex, formed fly, but how genetic and molecular biological experiments are used to determine such mechanisms, will find this book useful. The maternal systems that establish positional information in the egg cell, followed by the development of parasegments, and followed by expression of groups of cells, are described. It is shown that a large amount of genetic information is required to simply organize the embryo, besides building it. Many of the genes discussed have homologues in other higher animals such as vertebrates.

Good Book for research.......2000-03-25

I read this book when I was doing a project on drosophila menogaster, and this book really helped. There were some things that were hard to understand, but for the most part it was imformative and concise.

Drosophila: A Guide to Species Identification and Use

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Drosophila: A Guide to Species Identification and Use
Therese A. Markow
Manufacturer: Academic Press
ProductGroup: Book
Binding: Hardcover

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ASIN: 0124730523
Release Date: 2005-09-05

Book Description

Anyone wishing to tap the research potential of the hundreds of Drosophila species in addition to D.melanogaster will finally have a single comprehensive resource for identifying, rearing and using this diverse group of insects. This is the only group of higher eukaryotes for which the genomes of 12 species have been sequenced.

The fruitfly Drosophila melanogaster continues to be one of the greatest sources of information regarding the principles of heredity that apply to all animals, including humans. In reality, however, over a thousand different species of Drosophila exist, each with the potential to make their own unique contributions to the rapidly changing fields of genetics and evolution. This book, by providing basic information on how to identify and breed these other fruitflies, will allow investigators to take advantage, on a large scale, of the valuable qualities of these other Drosophila species and their newly developed genomic resources to address critical scientific questions.

* Provides easy to use keys and illustrations to identify different Drosophila species
* A guide to the life history differences of hundreds of species
* Worldwide distribution maps of hundreds of species
* Complete recipes for different Drosophila diets
* Offers an analysis on how to account for species differences in designing and conducting experiments
* Presents useful ideas of how to collect the many different Drosophila species in the wild

Imaginal Discs: The Genetic and Cellular Logic of Pattern Formation (Developmental and Cell Biology Series)

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Imaginal Discs: The Genetic and Cellular Logic of Pattern Formation (Developmental and Cell Biology Series)
Lewis I. Held Jr
Manufacturer: Cambridge University Press
ProductGroup: Book
Binding: Paperback

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ASIN: 0521018358

Book Description

This book examines the genetic circuitry of the well-known "fruit fly," tackling questions of cell assemblage and pattern formation, of the hows and the whys behind the development of the fly. After an initial examination of the proximity versus pedigree imperatives, the volume delves into bristle pattern formation and disc development, with entire chapters devoted to the leg, wing, and eye. Extensive appendices include a glossary of protein domains, catalogs of well-studied genes, and an outline of signaling pathways. More than 30 wiring diagrams among over 60 detailed schematics clarify the text.

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Biology of Drosophila
Milisav Demerec
Manufacturer: Cold Spring Harbor Laboratory Press
ProductGroup: Book
Binding: Hardcover

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ASIN: 0879694416

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